Production of human blood group B antigen epitope conjugated protein in Escherichia coli and utilization of the adsorption blood group B antibody

Background In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Although there are commercial columns to do this work, the application is still limited because of the high production cost. Results In this study, the PglB glycosylation pathway from Campylobacter jejuni was exploited to produce glycoprotein conjugated with Escherichia coli O86:B7 O-antigen, which bears the blood group B antigen epitope to absorb blood group B antibody in blood. The titers of blood group B antibody were reduced to a safe level without changing the clotting function of plasma after glycoprotein absorption of B antibodies in the plasma. Conclusions We developed a feasible strategy for the specific adsorption/removal of blood group antibodies. This method will be useful in ABOi organ transplantation and universal blood transfusion. Electronic supplementary material The online version of this article (doi:10.1186/s12934-016-0538-z) contains supplementary material, which is available to authorized users.


Background
The ABO blood group system is the most important blood type system in humans. Blood type incompatibility means the exposure of A or B antigen to a person who has antibodies against these antigens [1]. These antibodies act as haemagglutinins, which cause blood cells to clump and break apart, and can even cause death when large amounts of such cells are encountered after transfusion or organ transplant. Removal of anti-A and/or B antibodies from plasma is a promising method to overcome hyperacute rejection and allograft loss [2]. Several protocols have been employed to remove antibodies or antibody-producing cells in the process of ABOi organ transplantation [3], among which immunoadsorption has attracted more attention because of its specificity. The most commonly used immunoadsorbers are glycosorb columns with A/B blood group antigens linked to a sepharosematrix [4,5]. Unfortunately, A and B blood group antigens are difficult to acquire and immobilize [6].
At present, most A/B antigens used in glycosorb columns are synthesized by chemical methods or enzymatic synthesis. One of the most difficult steps in the chemical synthesis of well-defined oligosaccharide antigens is the stereospecific formation of glycosidic linkages between monosaccharide units [7]. Enzymatic synthesis utilizing the corresponding glycosyltransferase is limited by the availability of enzymes and the cost of activated sugar donors [8]. Accordingly, it is necessary to find a low-cost and highly-effective method to produce A/B antigens to remove anti-A/B antibodies from plasma. The O-antigen in Escherichia coli (E. coli) O86:B7 has been shown to possess high human blood group B activity because of the structural similarity between the O-antigen and human blood group B antigen epitope [9][10][11] (Fig. 1). Therefore, E. coli O86:B7 can be a potential cell factory of B antigens. We plan to obtain a type of glycoprotein loaded with this O-antigen that can be used to remove the A/B antibody from plasma. The oligosaccharyl transferase PglB from Campylobacter jejuni (C. jejuni) can transfer a wide range of polysaccharides from undecaprenyl-pyrophosphate (Und-PP) linked precursors to the asparagine of the consensus sequence D/E-X-N-Y-S/T (X, Y ≠ Proline) of the carrier protein in the periplasm [12,13]. The N-glycosylation pathway from C. jejuni in recombinant E. coli has been shown to be a simple method for producing glycoprotein [12].

Microbial Cell Factories
In our work, the N-glycosylation pathway of C. jejuni was used to produce glycoprotein conjugated with the O86 O-antigen (Fig. 2). The O86 O-antigen conjugatedprotein could adsorb anti-B antibody in the plasma, and the parameters of coagulation were not affected after the adsorbing process. Furthermore, it would have potential use in universal blood transfusion and may also be used in ABOi organ transplantation.

Results and discussion
The production and detection of MBP mut -OPS (O86:B7) bioconjugates To obtain glycoprotein loaded with OPS of O86:B7, PglB from C. jejuni was cloned into E. coli O86:B7 to transfer the O-antigen onto the protein, resulting in a kind of glycoprotein with OPS.
In E. coli, the OPS is transferred to lipid A by the WaaL enzyme to produce LPS. To effectively conjugate the OPS on a protein by PglB, the waaL gene was deleted from E. coli O86 using a λ-Red recombination system. The waaL gene deletion was confirmed using test primer pair t-waaL-F/t-waaL-R, which could amplify across the deletion area. Furthermore, ladder straps were observed on the SDS-PAGE gel of LPS from wild E. coli O86, while no straps were observed in the lane of the LPS extracted from E. coli O86 ΔwaaL, which indicated that the LPS of E. coli O86 ΔwaaL had a low degree of polymerization (Fig. 3). Therefore, we successfully obtained a strain of E. coli O86:B7 without the waaL gene.  Maltose-binding protein (MBP) was selected as a carrier protein for OPS with B antigen activity. MBP is expressed in the periplasm of E. coli by the malE gene [14], which is generally used as a tag for expression and purification of foreign recombinant proteins [15] with a Amylose-Resin column. MBP without an N-glycosylation site was modified to MBP mut with four consensus sequences at the C terminal for loading with blood group B antigen epitope by cloned PglB in E. coli O86 ΔwaaL. The glycosylated MBP mut (i.e. MBP mut -OPS) and unglycosylated MBP mut were purified from E. coli O86 ΔwaaL with or without the plasmid pACT3-PglB and identified by SDS-PAGE (Fig. 4a) and western blot using anti-His antibody (Fig. 4b), anti-MBP antibody (Fig. 4c) and anti-O86 OPS antibody (Fig. 4d), respectively.
When probed using the anti-His and anti-MBP antibody, a ladder of bands appeared on the blot when MBP mut was expressed in E. coli O86 ΔwaaL with PglB ( Fig. 4 lane 1), indicating that the protein was conjugated with multi-units of OPS (MBP mut -OPS), while unglycosylated MBP mut expressed in E. coli O86 ΔwaaL without PglB showed only one band with a molecular weight of 44 kDa, as expected ( Fig. 4 lane 2). During the process of western blot, O86 antiserum instead of monoclonal anti-O86 antibody was used to determine the O-antigen activity of MBP mut -OPS. Therefore, nonspecific reaction might occur in form of the lightgray band on the western blot. These results were consistent with the finding that OPS had typical variability of chain length with different degrees of polymerization [16]. Furthermore, conjugation of OPS on MBP mut was confirmed by MALDI-TOF mass spectrometry. As expected, the molecular weight of MBPmut -OPS (47,898.93 Da) was higher than that of MBP mut (44,017.39 Da) (Additional file 1: Figure S1). The average number of OPS repeat units in purified MBP mut -OPS was four based on the molecular weight of one repeat unit of OPS (894 Da) and MBP mut (44017 Da). Moreover, 1.5 mg MBP mut -OPS was purified from 1 L of fermentation, which provided a way to obtain a large yield of glycoproteins using E. coli O86. We believe that the yield can be improved after optimizations such as culture conditions, fermentation method. Still, the cost of this approach to produce B-antigen absorption material is much lower than tradition method which includes enzymatic synthesis of B-antigen saccharide using the corresponding glycosyltransferases because of the limited availability of enzymes, the high cost of activated sugar donors, etc.

Ability of MBP mut -OPS conjugates to bind to blood group B antibody
An ELISA assay was conducted to measure the ability of MBP mut -OPS conjugates to bind with anti-A/B antibody. Unglycosylated MBP mut without blood group activity was used as a negative control. The MBP mut -OPS can be recognized by the anti-B antibody (Fig. 5b), but not by the anti-A antibody (Fig. 5a). No binding between unglycosylated MBP mut and anti-A/B antibody was detected. These results suggested that MBP mut -OPS could bind anti-B antibody.

Specific absorption of blood group B antibody in the plasma
Based on the results of ELISA, MBP mut -OPS was applied to remove anti-B antibodies from blood group O and group A plasma as a B antigen. The removal rates of B antibody in plasma increased as increasing amounts of glycosylated MBP mut were added. The average blood group anti-B antibody titer of plasma samples of blood group O decreased from 64 to 4 (Fig. 6a) after treatment with MBP mut -OPS (320 μg/mL). A previous report indicated that the antibody titer of plasma samples ≤8 is compliant with the restricting final titer for undergoing surgery [17]. Analogously, upon evaluation of the plasma of blood group A, the titers of all samples decreased to a safe level of 4 after adsorption with a final concentration of 160 μg/mL MBP mut -OPS (Fig. 6b).
To investigate the effects of MBP mut -OPS on blood coagulation, the parameters of PT, APTT, TT, and Fib were detected within 4 h of blood withdrawn to citrate. None of the above parameters in the treated sample with MBP mut -OPS differed significantly from the control and levels remained normal (Fig. 6c). These results demonstrated that MBP mut -OPS could absorb anti-B antibody effectively and did not affect the coagulation properties of the plasma. Thus, the purified glycoprotein with blood group B epitope has great potential for clinical applications. MBP with O-antigen of E. coli O86 could be used to remove anti-B antibody from group O or A in emergency transfusions without strict matches. The produced MBP mut -OPS in E. coli O86 ΔwaaL will contribute significantly to the development of a method for universal blood transfusion. Furthermore, for actual clinical applications, the endotoxin of the glycoprotein produced from E. coli cells should be removed to a safety level, which will be taken into consideration in our following study.

Conclusions
This study successfully glycosylated MBP with B antigen using a novel one-shot approach. In the system, large quantities of glycoproteins are produced and have great potential for further clinical development in many fields including ABOi organ transplantation and universal blood transfusion. In addition, glycoprotein with blood group antigens could also be used as research tools or alternative drugs for infection or other diseases associated with blood group antigens. A similar strategy could extend to blood group A antigen since anti-A agglutinins were reported to be absorbed by an A active E. freundii [18].

Bacterial strains, plasmids and growth condition
Escherichia coli O86:B7 (ATCC 12701) was obtained from American Type Culture Collection (Rockville, MD). Commercially available IgM monoclonal anti-B antibody (obtained from clone HEB-29) was purchased from Merck Millipore (Billerica, USA). All strains and plasmids used in this study were listed in Additional file 1: Table S1. All strains were grown in Luria-Bertani broth (LB) at 37 °C. E. coli DH5α and O86 were used for plasmids cloning and glycoprotein expression experiments, respectively. Ampicillin (100 μg/mL), 50 μg/mL kanamycin and 34 μg/mL chloramphenicol were added to the media for selection as needed. Plasmids pKD4, pKD46 and pCP20 were used for the deletion of the gene coding for the O-polysaccharide ligase WaaL of E. coli O86. Plasmid pACT3 and pBAD24 were used for the expression of PglB and MBP protein, respectively.

Knockout of waaL gene of E. coli O86
The waaL gene of E. coli O86 was knocked out to obtain O86 ΔwaaL: FRT using λ-Red recombination system. Briefly, using plasmid pKD4 as template, the kanamycinresistant gene flanked by homologues of waaL gene was amplified by PCR with knockout primers. When induced by L-arabinose, plasmid pKD46 could express three recombinant proteins (Exo, Beta, Gam) of λ-prophage, which assisted the replacement of waaL gene with kanamycin-resistant gene. Subsequently, the kanamycin-resistant gene was eliminated by FLP-promoted recombination system using plasmid pCP20 and the E. coli O86 ΔwaaL was obtained successfully. The knockout primers (k-waaL-F, k-waaL-R) and test primers (t-waaL-F, t-waaL-R) used in the knockout experiments were listed in Additional file 1: Table S1. The extraction of LPS was carried out according to the instruction of LPS extraction kit (iNtRON Biotechnology, KOREA). The silver staining experiment was performed as reported previously [19].

Construction of recombinant plasmids
In order to ensure the successful glycosylation of MBP by PglB, the consensus sequence D-Q-N-A-T was repeated four times and inserted at the C terminal of MBP. Overlap PCR was used to amplify the malE mut gene with primers malE-F, malE-R1, malE-R2 and malE-R3 (Additional file 1: Table S1). Restriction sites for Sal I and Hind III at their 5′ ends of primers were used for the insertion of the modified gene into the vector pBAD24, and thus the plasmid pBAD24-malE mut was obtained with a 6× His tag (i.e. N-HHHHHH-C) between Sma I and Sal I of pBAD24 (Induced by L-arabinose). Likewise, the pglB gene from C. jejuni NCTC 11168 was inserted between Sma I and Sal I of plasmid pACT3 (Induced by IPTG) and the plasmid pACT3-PglB was obtained.

Glycoprotein expression and purification
The recombinant plasmids pBAD24-malE mut and pACT3-PglB were co-transformed into E. coli O86 ΔwaaL to obtain an engineering strain with the ability to produce MBP mut -OPS bioconjugates. Plasmid containing MBP mut gene was transformed into E. coli O86 ΔwaaL to produce unglycosylated MBP mut as a control. E. coli O86 ΔwaaL transferred with pBAD24-malE mut and pACT3-PglB was grown in 50 mL LB broth at 37 °C for 16 h, with shaking. Cultures were then inoculated 1/100 into 1 L TB broth and further grown at 37 °C with shaking until OD 600 reached 0.6. Subsequently, 0.1 % (w/v) L-arabinose and 50 μM IPTG were added to induce the expression of MBP and PglB, respectively. After further incubation at 28 °C for 6 h, 0.1 % (w/v) L-arabinose was added again for continuous induction of MBP.

Detection of purified glycoprotein
Western blotting was used to detect MBP and MBP mut -OPS expression. Samples were separated on 8 % SDSdenatured polyacrylamide gel and were then transferred onto nitrocellulose membrane. Membranes were blocked in 3 % BSA solution for 1 h at room temperature, and then were incubated with anti-hexahistine (anti-His) monoclonal antibody and anti-MBP monoclonal antibody (Beyotime Biotechnology, China), as well as anti-O86 O-antigen polyclonal antibody (Tianjin Biochip Corporation, China), respectively overnight at 4 °C. The secondary antibodies with a horseradish peroxidase (HRP) (Abcam, UK) were used subsequently. The image acquisition was finished by Flour ChemQ (Proteinsimple, US). MALDI-TOF result was analyzed by the MALDI-TOF mass spectrometer (AXIMA Confidence, SHIMAZU, Japan) with sinapic acid as the matrix (50 % ACN, 50 % H 2 O, 0.1 % TFA).

Binding ability measurement of glycoprotein and anti-B antibody
Polystyrene microtiter plates were coated by the purified proteins MBP/MBP mut -OPS from E. coli O86 at different concentration overnight at 4 °C. The plates were blocked with 2 % BSA in PBS buffer for 2 h at room temperature. After being washed three times with PBST (PBS, 0.05 % Tween-20), the plates were incubated with anti-B antibody diluted to 1:20 for 2 h, or with anti-A antibody (1:20) as control. After washing, the secondary antibody goat anti-mouse IgM conjugated to HRP (1:20,000) (Abcam, UK) was added and maintained for 1 h. Finally, the TMB substrate was used to develop the signal and 1 M HCl was used to terminate the reaction, and the OD was measured at 450 nm on Bio-Rad680 microplate reader (Hercules, California, USA).

Detection of the B antibody titer and coagulation parameters in the plasma
All blood samples, from 36 healthy people, were collected with citrate anticoagulation tubes, mixing, and were centrifuged at 1000g for 10 min to separate plasma. The plasma was divided two portions, one for the detection of B antibody titers, the other for coagulation analysis.
The B antibody titers in the plasma were measured with the polybrene test according to the instruction (Baso Biological Technology Corporation, Zhuhai, China). Briefly, twofold serial dilutions of plasma sample from 1:2 were made with normal saline for each tube. The same volume of 2 % type Bred blood cells were added to each tube and mixed thoroughly. Low ionic medium, polybrene reagent and resuspending were added subsequently and operated based on the instruction, and the smallest dilution which could still agglutinate erythrocyte was determined as the endpoint, and its reciprocal was considered as the titer of the sample plasma.
In order to detect the effects of proteins MBP mut -OPS on blood clotting function, coagulation parameters of the samples treated/pre-treated with MBP mut -OPS were measured with fully automatic blood coagulation analyzer ACL7000 (BECKMAN, USA).

Adsorption of blood group B antibody in the plasma
Aliquots of plasma samples of 800 μL were mixed with final concentration of 0, 80, 160 and 320 μg/mL MBP mut -OPS, respectively. After incubation at room temperature for 1 h, the B antibody titer and clotting parameters in the plasma were detected as above methods.

Statistical analysis
The statistical analyses and figures were generated by GRAPHPAD PRISM software version 5.0. Data were shown as mean ± standard deviation (SD). The difference between two groups was compared by t test. For multiple comparisons, One-way ANOVA was used. A probability (P) value ≤ 0.05 was considered statistically significant.