The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum

Background Edible mushrooms are delicious in flavour and rich in high-quality protein and amino acids required by humans. A transcription factor, general control nonderepressible 4 (GCN4), can regulate the expression of genes involved in amino acid metabolism in yeast and mammals. A previous study revealed that GCN4 plays a pivotal role in nitrogen utilization and growth in Ganoderma lucidum. However, its regulation is nearly unknown in mushrooms. Results In this study, we found that the amino acid contents reached 120.51 mg per gram of mycelia in the WT strain under 60 mM asparagine (Asn) conditions, but decreased by 62.96% under 3 mM Asn conditions. Second, silencing of gcn4 resulted in a 54.2% decrease in amino acid contents under 60 mM Asn, especially for the essential and monosodium glutamate-like flavour amino acids. However, these effects were more pronounced under 3 mM Asn. Third, silencing of gcn4 markedly inhibited the expression of amino acid biosynthesis and transport genes. In addition, GCN4 enhanced the tricarboxylic acid cycle (TCA) and glycolytic pathway and inhibited the activity of target of rapamycin complex 1 (TORC1), thus being beneficial for maintaining amino acid homeostasis. Conclusion This study confirmed that GCN4 contributes to maintaining the amino acid contents in mushrooms under low concentrations of nitrogen. In conclusion, our study provides a research basis for GCN4 to regulate amino acid synthesis and improve the nutrient contents of edible mushrooms. Supplementary Information The online version contains supplementary material available at 10.1186/s12934-023-02213-z.

The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum Background Edible mushrooms are considered a so-called 'superfood' or functional food due to their umami flavour and the presence of triterpenoids, polysaccharides, proteins, peptides, and phenolic compounds [1].Proteins in edible mushrooms are rich and the content is as high as 18.87-36.96% of the dry weight, with a complete profile of essential amino acids (EAAs) required for adults [2].If fungal protein is obtained by fermentation, it can replace 20% of global beef consumption and reduce deforestation and carbon dioxide emissions by 56% [3].Plant proteins do not meet human dietetic requirements due to the lack of some essential amino acids.Therefore, mushroom proteins have the potential to serve as a viable supplement to animal and plant proteins [4].
Amino acid composition, especially the content of essential amino acids is a reliable indicator of protein quality [2].Edible fungal amino acids not only contain the complete amino acids profile but also have unique flavours and functions [5][6][7].The content of essential amino acids in Calvatia gigantea accounts for more than 56% of the content of free amino acids [8].As an excellent source of the essential amino acids, L-lysine content is high in Flammulina velutipes [9].The amino acids in mushrooms are divided into four groups: the umami taste amino acids, the sweet amino acids, the bitter amino acids and the tasteless amino acids [5,10].Among them, aspartic acid and glutamic acid, belonging to the monosodium glutamate-like (MSG-like) component class, are relatively abundant in most mushrooms, and provide the umami or palatable flavour of mushrooms [11,12].Therefore, the content and composition of amino acids are important indicators to evaluate the nutrition and flavor of edible mushrooms.
In edible mushrooms, changes in environmental or cultivation conditions, as well as the expression of some key genes involved in amino acid synthesis, affect the amino acid contents.Using the wheat stalk as the growth substrate led to good essential amino acid patterns in Pleurotus ostreatus [13,14].High sawdust content or a high C/N value were beneficial to high levels of protein and amino acids in P. eryngii [15].Blue light and chitosan treatment increased the content of lysine in F. velutipes [16].In addition, genes involved in the biosynthesis of specific critical amino acids, such as lysine in F. velutipes and cysteine in Lentinus edodes, were studied.Overexpression of sdh, the last step of fungal lysine biosynthesis, significantly increased the lysine content in F. velutipes [17].The formation of unique aromas in L. edodes is related to the synthesis of cysteine.A cysteine synthesis gene, cysteine sulfoxide lyase gene, has been found to affect the formation of organosulfur compounds in L. edodes [18].Although these studies have focused on the amino acid biosynthesis in edible mushrooms, there are few investigations on the regulation mechanism of amino acid contents.
Organisms can maintain the intracellular amino acid homeostasis in many aspects, including regulating the activities of amino acid transporters, de novo synthesis and degradation of proteins, in which signals, genes and pathways participate [19].Gcn4, a basic leucine zipper (bZIP) transcription factor, is the master transcriptional activator of genes for amino acid biosynthesis in fungi [20,21].Analysis of cDNA microarray expression profiles in Saccharomyces cerevisiae revealed that GCN4 regulates the expression of more than 30 amino acid biosynthesis genes [22,23].CpcA (homologue of GCN4) in Aspergillus fumigatus was the transcriptional activator of the cross-pathway control (CPC) system of amino acid biosynthesis [24].In addition, the tryptophan synthaseencoding gene (trpB) and arginine synthase-encoding gene (argB) in A. nidulans were regulated by CpcA [25,26].However, the functions of GCN4 have rarely been reported in basidiomycetes, especially in edible mushrooms.
As a representative edible and medicinal mushroom, Ganoderma lucidum has attracted extensive attention and research interest due to its important pharmacological and high economic value [27], the complete genome sequence information [28] and the well-establishment of the genetic transformation system [29].G. lucidum is susceptible to a low nitrogen during the late growth and development stages.In our previous study, we found that GCN4 was activated to maintain growth under the lownitrogen conditions [30].Here we found that GCN4 was important for the intracellular amino acid homeostasis, especially the MSG-like taste amino acid and the essential amino acid contents.Furthermore, GCN4 promoted the expression of the genes involved in the amino acid homeostasis under the low concentrations of nitrogen.In addition, GCN4 increased the TCA cycle and glycolysis pathway to provide precursors for amino acid biosynthesis, and inhibited the activity of target of rapamycin complex 1 (TORC1) to reduce the utilization of amino acids.In conclusion, our findings provide a reference for the exploration of intracellular amino acid regulatory networks in edible mushrooms.

The amino acid contents of Ganoderma lucidumunder lownitrogen concentrations
Nitrogen is the main macronutrient for fungal structure and energy requirements.G. lucidum suffers nitrogenlimiting conditions during its growth.To determine the specific mechanism, we screened for dominant nitrogen sources for the growth of G. lucidum mycelia.The G. lucidum had good growth status when asparagine (Asn) was used as the sole nitrogen source (Fig. S1).Therefore, low concentrations of Asn were utilized to mimic nitrogen-limiting conditions and explored the intracellular amino acid content.The content of each detected amino acid decreased under low-nitrogen conditions (Table 1), compared with that under the high-nitrogen conditions, except for threonine.As shown in Fig. 1A, the amino acid content reached 120.51 mg/g in the WT strain under the 60 mM Asn condition.However, the content of amino acids under the 3 mM Asn decreased by 62.96% compared with that under 60 mM Asn (Fig. 1A).Under the low concentration of nitrogen, the contents of essential amino acids decreased by 52.63% compared with the high concentration of nitrogen (Fig. 1B).The contents of MSG-like, sweet, bitter and tasteless amino acids under 3 mM Asn decreased by 79.06%, 14.65%, 47.35% and 44.23%, respectively, compared with those under 60 mM Asn (Fig. 1C-F).Taken together, these results showed that a high concentration of nitrogen benefits the amino acid accumulation, while a low concentration of nitrogen resulted in a decrease in amino acid contents in G. lucidum.

Amino acid contents in gcn4-silenced strains under lownitrogen concentrations
Our previous study found that GCN4 was highly induced in response to a low concentration of nitrogen condition in G. lucidum.To assess the regulation of GCN4 on amino acid contents in G. lucidum, we examined the amino acid contents in gcn4-silenced strains.The content of almost every amino acid in the gcn4-silenced strains was significantly lower than that in the WT strain under the different nitrogen concentrations (Table 1).Silencing of gcn4 resulted in a 54.2% decrease under the 60 mM Asn condition.However, under the 3 mM Asn condition, the contents of amino acids decreased by 59.8% and 62.3% in the gcn4i-1 and gcn4i-22 strains, respectively, compared with those in WT (Fig. 1A).Silencing of gcn4 significantly decreased essential and tasteless amino acids under the 3 mM Asn (Fig. 1B, F).The contents of MSG-like taste amino acids decreased by 79.8% and 88.3% in the gcn4i-1 and gcn4i-22 strains compared with those in the WT but decreased by 69.8% and 68.3% under 60 mM Asn (Fig. 1C).The sweet amino acids in the gcn4silenced strains showed no significant change compared with those in the WT under both conditions (Fig. 1D).In conclusion, GCN4 facilitated the accumulation of amino acids, especially the essential and MSG-like taste amino acids, under low-nitrogen conditions.

The expression of amino acid metabolism-related genes under low-nitrogen concentrations
Intracellular amino acids are obtained through many pathways, and GCN4 is one of the master regulators of genes involved in amino acid transport and biosynthesis [19].We further investigated the expression of genes related to amino acid metabolism.Glutamic acid and aspartic acid are the most abundant amino acids in mushrooms, as well as in G. lucidum (Table 1).Therefore, the expression of genes including the asparagine synthetase gene (asns), glutamine synthetase gene (gs), glutamate synthase gene (gogat), glutamic oxaloacetic-transaminase gene (got1 and got2) and glutamic-pyruvic transaminase gene (gpt) were detected.All these genes in the WT strain were significantly induced under 3 mM Asn, especially the asns gene, with 2.41-fold high expression than that under 60 mM Asn (Fig. 2A).Silencing of gcn4 markedly inhibited the expression of almost all these genes, with a reduction of approximately 58.71-90.83%compared with WT under 3 mM Asn.Under 60 mM Asn, they decreased by 24.64-62.88% in gcn4-silenced strains compared with WT (Fig. 2A-F).However, the expression of gogat was unchanged in both the WT and in the gcn4-silenced strains (Fig. 2C).Furthermore, the expression of 7 amino acid transport genes (Gl23068, Gl29937, Gl20736, Gl21744, Gl23271, Gl28933, and Gl23088) was decreased by 71.29-92.59% in the gcn4-silenced strains under 3 mM Asn (Fig. 2G-M) and decreased by 22.84-60.1% under 60 mM Asn.These results indicated that silencing of gcn4 decreased the expression of genes related to amino acid metabolism, especially under lownitrogen conditions.

Effect of the TCA and glycolysis pathways in gcn4-silenced strains under low-nitrogen concentrations
Amino acids are also replenished through the TCA cycle and glycolysis pathway [31,32].Therefore, we further investigated the effect of GCN4 on the TCA cycle and glycolysis pathway.As shown in Fig. 3, compared with 60 mM Asn, the activities of mitochondrial isocitrate dehydrogenase (IDH) and α-ketoglutarate dehydrogenase (KGDH) in WT were significantly upregulated by 1.9 and 2.4-fold, respectively (Fig. 3A).The activities of hexokinase (HK), pyruvate kinase (PK), and phosphofructokinase (PFK) increased 1.65-, 1.52-and 2.2-fold, respectively, compared to 60 mM Asn (Fig. 3C).These results suggested that low-nitrogen conditions promote the TCA cycle and glycolysis pathway in G. lucidum.Furthermore, under 3 mM Asn, after silencing gcn4, the enzyme activities of ICDH and KGDH decreased by 56% and 92.1%, respectively, compared with the WT strain.This treatment also led to 54.63%, 83.4% and 62.39% decreases in PFK, HK and PK activities, respectively.However, the activities of ICDH and three key enzymes involved in the glycolysis pathway showed no difference between the WT and gcn4-silenced strains under 60 mM Asn (Fig. 3A, B).The expression of genes in gcn4-silenced strains corresponded to the enzyme activities and exhibited a significant decrease of 35.38-83.15%under 3 mM Asn (Fig. 3B and D).The above results suggest that GCN4 enhances the TCA cycle and glycolysis pathway under low-nitrogen conditions.

The activity of TORC1 in gcn4-silenced strains under lownitrogen concentrations
The TORC1 plays a central role in sensing and regulating the metabolism and availability of amino acids [33].To comprehensively analyse the regulation of amino acid homeostasis by GCN4, the activity of TORC1 (characterized by the phosphorylation state of S6K) was tested (Fig. 4A).The phosphorylation level of S6K in the WT strain significantly decreased by 66.4% at 3 mM Asn compared with 60 mM Asn.However, silencing of gcn4 increased S6K phosphorylation, with 71.4% and 22% increases under 3 mM Asn and 60 mM Asn, respectively (Fig. 4B).This result suggests that GCN4 exerts an inhibitory effect on TORC1 activity, especially under lownitrogen conditions.

Discussion
Researchers have been committed to finding low-cost sources of protein that can replace those of animal origin [4].Compared with plant or animal protein, edible mushroom production is faster and less expensive.In addition, mushrooms provide not only a significant protein content with a complete essential amino acid profile, but Fig. 2 Involvement of GCN4 in regulating intracellular amino acid biosynthesis-related genes under low-nitrogen conditions.Relative expression of genes related to amino acid synthesis and transport in WT and gcn4-silenced strains under 3 mM and 60 mM Asn conditions.Expression of the asparagine synthetase gene (asns), glutamine synthetase gene (gs), glutamate synthase gene (gogat), glutamic oxaloacetic transaminase genes (got1 and got2) and glutamic-pyruvic transaminase gene (gpt), as well as 7 that of amino acid transport genes (Gl23068, Gl29937, Gl20736, Gl21744, Gl23271, Gl28933, Gl23088), were determined.Expression levels of genes in the WT strain cultured under 60 mM Asn were defined as 1.The data are presented as the mean ± SD (n = 3).Statistical significance is represented by different letters corresponding to P < 0.05 based on Tukey′s test also the flavoring amino acids [2,34].Therefore, mushrooms protein can be used as a potential supplement to meat or plant proteins.Although the effects of environmental or cultivation conditions on the amino acid contents have been investigated, the regulation of amino acid metabolism in mushrooms is unclear.Our study provides new insight into the molecular mechanism of GCN4 in regulating amino acid metabolism in the mushroom G. lucidum.Under low-nitrogen conditions, GCN4 regulated the expression of genes involved in amino acid synthesis and transporter genes and promoted the glycolysis pathway and TCA cycle to provide precursors for amino acid biosynthesis.Additionally, the utilization of amino acids by TORC1 was hindered by GCN4, thereby maintaining the amino acid contents under low-nitrogen conditions in G. lucidum (Fig. 5).Taken together, the results of this study explain the regulatory mechanism of GCN4 in amino acid metabolism and provides the basis for further studies on the regulation of amino acid metabolism in edible mushrooms.Nitrogen is a crucial nutrient for biological growth and development, playing a pivotal role in the synthesis of amino acids.In Schizophyllum commune and Coprinus cinereus, the mycelial growth and free amino acid concentration of mycelia grown on the nitrogen-rich medium (6.6 mM L-asparagine) increased compared with that on the nitrogen-poor medium (0.06 mM L-asparagine) [35,36].G. lucidum showed higher cell dry weight and ganoderic acids under organic nitrogen sources (glutamine, asparagine and glycine) [37].Our study also found that using asparagine as the sole nitrogen source led to good growth and significantly activated GCN4 expression (Fig. S1).In addition, the amino acid contents under low-nitrogen conditions decreased by 62.96% compared with those under high-nitrogen conditions in G. lucidum.Low-nitrogen conditions activate a key transcription factor GCN4 to maintain mycelial growth and resistance to intracellular oxidative stress in G. lucidum [30].Here, silencing of gcn4 significantly reduced the intracellular amino acid contents and inhibited amino acid biosynthesis, which means that GCN4 contributes to maintaining intracellular amino acid homeostasis under low-nitrogen conditions in G. lucidum.
The functions of GCN4 in fungal growth and regulating genes involved in amino acid homeostasis are well known in some filamentous fungi and mammals [38][39][40][41].In addition, the gene expression of specific key amino acid synthesis genes (ASNS, alanine aminotransferase 2, PSAT, serine hydroxymethyl transferase 2, pyrroline-5-carboxylate reductase and glutamate-oxaloacetate transaminase) genes in mammals was reported widely regulated by ATF4/GCN4 [19], Additionally, in yeast, the regulation of specific amino acid biosynthesis genes, including histidine and isoleucine/valine synthesis genes such as his4, his3, ilv1, and ilv2 has also been observed [22].Here, we examined the expression of genes involved in amino acid synthesis in gcn4-silenced.In G. lucidum, GCN4 enhanced the expression of asns, which is the typical target gene of GCN4 in mammals [41].In addition, GOT coordinates carbon and nitrogen metabolism in organisms [42] and affects the intracellular glutamate content [43], which is the key metabolite in the intracellular amino acid biosynthesis [44].In our study, transaminase genes (got1 and got2) were regulated by GCN4.These results were consistent with previous studies showing that GCN4 is the master regulator of gene expression in amino acid synthesis in yeast [20].Our study demonstrated for the first time in edible mushrooms that GCN4 regulated amino acid contents, as well as the expression of amino acid biosynthesis and transporter genes, which was important for improving the nutritional value of mushrooms.
The intermediate metabolites of carbon metabolism, such as pyruvate, oxaloacetate (OAA), and α-ketoglutarate (α-KG), are the respective precursors for the biosynthesis of amino acids [44,45].Here, we found that the activities of key enzymes and the expression of key genes in the TCA cycle and glycolysis were significantly upregulated when the mycelia were cultured under low-nitrogen conditions, which suggested that the TCA cycle and glycolysis might be activated to promote the synthesis of intracellular amino acids in response to nitrogen-limiting conditions.However, this upregulation disappeared in gcn4-silenced strains, suggesting that the GCN4 was involved in promoting the TCA cycle and glycolysis to provide precursors for amino acid synthesis and ultimately maintain intracellular amino acid homeostasis under nitrogen-limitation conditions.In addition, TORC1 is the central energy hub for sensing and regulating the metabolism and availability of amino acids [46].Here, we found that TORC1 was also involved in the regulation of amino acids by GCN4.The phosphorylation of Fig. 5 Schematic indicating that GCN4 regulates intracellular amino acid contents under low-nitrogen conditions.Under low-nitrogen conditions, GCN4 promotes the synthesis of intracellular amino acids through the transcriptional regulation of genes related to amino acid synthesis.In addition, GCN4 activates the TCA cycle and glycolytic pathway and inhibits the activity of TORC1 to replenish intracellular amino acids S6K was significantly reduced under nitrogen-limitation conditions, while silencing of gcn4 activated phosphorylation of S6K, which is consistent with previous studies in yeast showing that the existence of GCN4 inhibited the phosphorylation of S6K [47].Our results indicated that GCN4 was involved in the inhibition of TORC1 activity under low-nitrogen conditions.Considering that TORC1 recognizes and uses amino acids and activates S6K to regulate the synthesis of ribosomal proteins, as well as translationally regulated proteins [48], we hypothesized that GCN4 negatively regulates TORC1 under lownitrogen conditions to inhibit protein synthesis, provide sufficient amino acids for cells, and ultimately comprehensively regulate amino acid homeostasis.

Fungal strains and culture conditions
The G. lucidum strain (ACCC53264) was obtained from the Agricultural Culture Collection of China (ACCC).The gcn4-silenced strains were obtained from our previous study [30].The wild-type (WT) and gcn4-silenced strains of G. lucidum were cultivated in the complete yeast medium (CYM) which containing 2% glucose, 1% maltose, 0.46% KH 2 PO 4 , 0.2% yeast extract, 0.2% tryptone, and 0.05% MgSO 4 •7H 2 O. Cultivation under nitrogen limitation conditions was performed according to the method previously described.Cultures with different nitrogen sources were prepared by replacing the yeast extract and tryptone of CYM with 3 mM or 60 mM Asn [31,33].

qRT-PCR analysis
The strains cultivated at 28 °C were collected for the total RNA extraction using RNAiso Plus reagent (TaKaRa, Dalian, China).cDNA was obtained by reverse transcription using a 5x All-In-One RT MasterMix kit (Vazyme, Nanjing, China).qRT-PCR was performed using EvaGreen 2X qPCR MasterMix (Vazyme, Nanjing, China).Gene expression was normalized against the 18 S reference gene using the primers listed in Table S1.

Enzymatic activity assays
The enzyme activities of mitochondrial isocitrate dehydrogenase (IDH), α-ketoglutarate dehydrogenase (KGDH), hexokinase (HK), pyruvate kinase (PK), and phosphofructokinase (PFK) were analyzed using detection kit (Solarbio, Beijing, China), respectively, according to the manufacturers' protocols.All values were normalized to the total protein levels.The protein content was quantified by the Bradford Protein Assay Kit (Sangon, Shanghai, China).All these enzymatic activities in the control group were normalized to 1.0.

Statistical analysis
Statistical analysis in this study was performed using GraphPad Prism 6 (GraphPad Software, San Diego, USA).The data from at least three independent sample measurements were averaged, and the values shown are the means and standard deviations (SDs).Data analyses using Student's t test were used for two-sample comparisons, and one-way analysis of variance (ANOVA) with Duncan's posttest was used for multiple comparisons.
edible mushrooms suitable for factory cultivation" (No. 2022YFD1200605), the National Natural Science Foundation of China (No. 31900065 and 32302631), and the earmarked fund for CARS20.

Fig. 1
Fig. 1 Involvement of GCN4 in regulating intracellular amino acid contents.Intracellular amino acid contents (A), essential amino acid contents (B), MSGlike flavour amino acid contents (C), sweet flavour amino acid contents (D), bitter flavour amino acid contents (E) and flavourless amino acid contents (F) in WT and gcn4-silenced strains.MSG-like flavour amino acids include aspartic acid and glutamic acid.Sweet flavour amino acids include threonine, serine, glycine and alanine.Bitter flavour amino acids include valine, methionine, isoleucine, leucine, phenylalanine, histidine, arginine and tryptophan.Flavourless amino acids include cystine, tyrosine and lysine.The data are presented as the mean ± SD (n = 3).Statistical significance is represented by different letters corresponding to P < 0.05 based on Tukey′s test

Fig. 4 Fig. 3
Fig.4GCN4 inhibits TORC1 activity under low-nitrogen conditions.Western blot analysis of S6K phosphorylation levels in the WT and gcn4-silenced strains cultured under 3 mM and 60 mM Asn, with histograms showing the relative ratio of p-S6K/Total-S6K.The p-S6K/Total-S6K ratio in the WT strain grown under 60 mM Asn was defined as 1.The data are presented as the mean ± SD (n = 3).Statistical significance is represented by different letters according to Tukey′s test (P < 0.05)

Table 1
The amino acid contents of WT and gcn4-silenced strains under different nitrogen conditions