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  1. Actinomycetes (Actinobacteria) are highly attractive as cell factories or bioreactors for applications in industrial, agricultural, environmental, and pharmaceutical fields. Genome sequencing of several species o...

    Authors: Nobutaka Nakashima, Yasuo Mitani and Tomohiro Tamura
    Citation: Microbial Cell Factories 2005 4:7
  2. The permanently impaired protein folding during recombinant protein production resembles the stress encountered at extreme temperatures, under which condition the putative holding chaperones, IbpA/IbpB, play a...

    Authors: Ha LeThanh, Peter Neubauer and Frank Hoffmann
    Citation: Microbial Cell Factories 2005 4:6
  3. Carbon dioxide fixation bioprocess in reactors necessitates recycling of D-ribulose1,5-bisphosphate (RuBP) for continuous operation. A radically new close loop of RuBP regenerating reactor design has been prop...

    Authors: Debojyoti De, Debajyoti Dutta, Moloy Kundu, Sourav Mahato, Marc T Schiavone, Surabhi Chaudhuri, Ashok Giri, Vidya Gupta and Sanjoy K Bhattacharya
    Citation: Microbial Cell Factories 2005 4:5
  4. Hydrocarbon-degrading biofilms in the treatment of contaminated groundwaters have received increasing attention due to the role played in the so-called "biobarriers". These are bioremediation systems in which ...

    Authors: Alessandra Di Lorenzo, Mario Varcamonti, Palma Parascandola, Rodolfo Vignola, Adriano Bernardi, Pasquale Sacceddu, Raffaello Sisto and Elisabetta de Alteriis
    Citation: Microbial Cell Factories 2005 4:4
  5. Lactococcus lactis, the model lactic acid bacterium (LAB), is a food grade and well-characterized Gram positive bacterium. It is a good candidate for heterologous protein delivery in foodstuff or in the digestive...

    Authors: Yves Le Loir, Vasco Azevedo, Sergio C Oliveira, Daniela A Freitas, Anderson Miyoshi, Luis G Bermúdez-Humarán, Sébastien Nouaille, Luciana A Ribeiro, Sophie Leclercq, Jane E Gabriel, Valeria D Guimaraes, Maricê N Oliveira, Cathy Charlier, Michel Gautier and Philippe Langella
    Citation: Microbial Cell Factories 2005 4:2
  6. Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty. The lack of a published genome sequence re...

    Authors: Michael Sauer, Paola Branduardi, Brigitte Gasser, Minoska Valli, Michael Maurer, Danilo Porro and Diethard Mattanovich
    Citation: Microbial Cell Factories 2004 3:17
  7. Antibody fragments are molecules widely used for diagnosis and therapy. A large amount of protein is frequently required for such applications. New approaches using folding reporter enzymes have recently been ...

    Authors: Pascal Philibert and Pierre Martineau
    Citation: Microbial Cell Factories 2004 3:16
  8. Sugar binding proteins and binders of intermediate sugar metabolites derived from microbes are increasingly being used as reagents in new and expanding areas of biotechnology. The fixation of carbon dioxide at...

    Authors: Sourav Mahato, Debojyoti De, Debajyoti Dutta, Moloy Kundu, Sumana Bhattacharya, Marc T Schiavone and Sanjoy K Bhattacharya
    Citation: Microbial Cell Factories 2004 3:7
  9. The genome sequence analysis of Thermus thermophilus HB27, a microorganism with high biotechnological potential, has recently been published. In that report, the chromosomal and the megaplasmid sequence were comp...

    Authors: Efthimia E Lioliou, Anastasia A Pantazaki and Dimitrios A Kyriakidis
    Citation: Microbial Cell Factories 2004 3:5
  10. The proper functioning of extracytoplasmic proteins requires their export to, and productive folding in, the correct cellular compartment. All proteins in Escherichia coli are initially synthesized in the cytopla...

    Authors: Marika Miot and Jean-Michel Betton
    Citation: Microbial Cell Factories 2004 3:4
  11. Bacillus cereus constitutes a significant cause of acute food poisoning in humans. Despite the recent development of different detection methods, new effective control measures and better diagnostic tools are req...

    Authors: Magdalena Gabig-Ciminska, Heiko Andresen, Joerg Albers, Rainer Hintsche and Sven-Olof Enfors
    Citation: Microbial Cell Factories 2004 3:2
  12. A protein quality control system, consisting of molecular chaperones and proteases, controls the folding status of proteins and prevents the aggregation of misfolded proteins by either refolding or degrading a...

    Authors: Jimena Weibezahn, Bernd Bukau and Axel Mogk
    Citation: Microbial Cell Factories 2004 3:1
  13. A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut+ strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. A recombinant fusion ...

    Authors: Mehmedalija Jahic, Fredrik Wallberg, Monika Bollok, Percival Garcia and Sven-Olof Enfors
    Citation: Microbial Cell Factories 2003 2:6
  14. The bioavailability of iron is quite low since it is usually present as insoluble complexes. To solve the bioavailability problem microorganisms have developed highly efficient iron-scavenging systems based on...

    Authors: Francisco J Flores, Javier Rincón and Juan F Martín
    Citation: Microbial Cell Factories 2003 2:5
  15. A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were...

    Authors: Jari Rautio, Kim Bundvig Barken, Juhani Lahdenperä, Antje Breitenstein, Søren Molin and Peter Neubauer
    Citation: Microbial Cell Factories 2003 2:4
  16. In contrast to bioreactors the metabolites within the microbial cells are converted in an impure atmosphere, yet the productivity seems to be well regulated and not affected by changes in operation variables. ...

    Authors: Sumana Bhattacharya, Subhra Chakrabarti, Amiya Nayak and Sanjoy K Bhattacharya
    Citation: Microbial Cell Factories 2003 2:3
  17. Use of lactose-rich concentrates from dairy processes for the induction of recombinant gene's expression has not received much attention although they are interesting low cost substrates for production of reco...

    Authors: Mikko I Viitanen, Antti Vasala, Peter Neubauer and Tapani Alatossava
    Citation: Microbial Cell Factories 2003 2:2
  18. In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody...

    Authors: Vivi Joosten, Christien Lokman, Cees AMJJ van den Hondel and Peter J Punt
    Citation: Microbial Cell Factories 2003 2:1
  19. The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans. CGTases are produced by a variety o...

    Authors: Adriana M Rosso, Susana A Ferrarotti, Norberto Krymkiewicz and Bclara Nudel
    Citation: Microbial Cell Factories 2002 1:3
  20. Applications of bacteriocins as food preservatives have been so far limited, principally because of their low antimicrobial activity in foods. Nisin is the only bacteriocin of significant use, but applications...

    Authors: Luca Martirani, Mario Varcamonti, Gino Naclerio and Maurilio De Felice
    Citation: Microbial Cell Factories 2002 1:1

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