Articles

Kinetics of aggregation and structural properties of proteins in inclusion bodies studied by Fourier transform infrared spectroscopy

Authors: Antonino Natalello, Diletta Ami, Pietro Gatti-Lafranconi, Ario de Marco, Marina Lotti and Silvia Maria Doglia

Analysis and characterization of different preparations of recombinant human follicle stimulating hormone (hFSH) and of its subunits

Authors: Maria Teresa CP Ribela, Renan F Loureiro, João E Oliveira, Cristiane M Carvalho, Cibele N Peroni and Paolo Bartolini

ColE1 derived RNA I as a key molecule in a novel antibiotic free plasmid addiction system

Authors: Irene Pfaffenzeller, Gerald Striedner, Karl Bayer and Reingard Grabherr

Recombinant lipase immobilised in the cell wall of Bacillus halodurans Alk 36 exploiting the FliC protein

Authors: Michael Crampton, Erika du Plessis, Santosh Ramchuran, Eldie Berger and Maureen Louw

A regulatory acceptable alternative to E. coli: high yield recombinant protein production using the Lactococcus lactis P170 expression system combined with "Reverse electro enhanced dialysis" (REED) for lactate control

Authors: Søren M Madsen, Astrid Vrang, Lars H Pedersen, Sean A MacDonald, Jens-Ulrik Rype and Arvid Garde

N-terminally acetylated tropomyosin generated in E. coli by coexpression of the S. cerevisiae NatB acetylation complex shows functional properties in vitro

Authors: Robin Maytum and Manfred Konrad

Bacillus megaterium as a recombinant protein production host

Authors: Yang Yang, Marco Malten, Rebekka Biedendieck, Wei Wang, Dieter Jahn and Wolf-Dieter Deckwer

Automation for higher throughput in protein expression: visions, facts and fictions

Authors: Marion Mahnke, Jean Marc Schlaeppi, Yann Pouliquen, Louise Barys, Catherine Rolvering, Mario Henke, Rita Schmitz, Sabine Geisse and Frank Kolbinger

Limitations using GFP as a protein expression reporter in Pichia pastoris

Authors: Anna Surribas, David Resina, Pau Ferrer and Francisco Valero

Application of a genome-scale metabolic model to the inference of nutritional requirements and metabolic bottlenecks during recombinant protein production in Escherichia coli

Authors: Sónia Carneiro, Isabel Rocha and Eugénio Ferreira

HYBMFA: a bioinformatics' tool for batch-to-batch bioprocess optimisation supported by elementary flux analysis

Authors: Ana Teixeira, Carlos Alves, Paula Alves, Manuel Carrondo and Rui Oliveira

Recombinant expression of disulfide-rich proteins: carboxypeptidase inhibitors as model proteins

Authors: Laura Sanglas, Sílvia Bronsoms, Joan L Arolas, Julia Lorenzo and Francesc X Aviles

Structural biology of Helicobacter pylori type IV secretion system

Authors: Alessandro Angelini, Laura Cendron, Anke Seydel, Nicola Barison, Tommaso Tosi, Roberto Battistutta and Giuseppe Zanotti

Efficient, antibody-mediated allosteric activation of an immobilized, E. coli beta-galactosidase recombinant biosensor

Authors: Rosa M Ferraz, Anna Arís, Gregorio Álvaro and Antonio Villaverde

Design of improved membrane protein production experiments in yeast: quantitation of the host response

Authors: Nicklas Bonander, Kristina Hedfalk, Christer Larsson, Petter Mostad, Celia Chang, Lena Gustafsson and Roslyn M Bill

Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

Authors: Georg Hinterkörner, Gudrun Brugger, Dethardt Müller, Friedemann Hesse, Renate Kunert, Hermann Katinger and Nicole Borth

Recombinant protein expression system in cold loving microorganisms

Authors: Rosanna Papa, Valentina Rippa, Giovanni Sannia, Gennaro Marino and Angela Duilio

Use of a "universal" yeast vector (CoMed™) system for the production of proteins in Hansenula polymorpha and Arxula adeninivorans

Authors: Gerd Gellissen, Gerhard Steinborn and Gotthard Kunze

A novel yeast expression system based on a hormone-induced transcriptional cascade

Authors: María José Quintero, Miguel Arévalo-Rodríguez, Ángel Cebolla and Sebastián Chávez

Determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor "indicator microorganism"

Successful application of growth inhibition techniques for quantitative determination of bacteriocins relies on the sensitivity of the applied indicator microorganism to the bacteriocin to which is exposed. Ho...

Authors: Maria Papagianni, Nicholaos Avramidis, George Filioussis, Despina Dasiou and Ioannis Ambrosiadis

Impact of high throughput technology on recombinant protein production

Authors: Jo J Jones, Angela M Bridges, Andrew P Fosberry, Jonathan Bullock, Ian Hudson, Richard M Hall, Owen Jenkins and Jeffrey A Cole

Set up and optimization of a fermentation protocol for the production of a human antibody fragment (Fab') express in E. coli. Pre-pilot and cGMP pilot scale studies

Authors: E Riscaldati, A Ciabini, A Baccante, D Moscatelli, MF Errichetti, A Colagrande, S Cencioni, F Marcocci, V Di Cioccio, L Di Ciccio, M Allegretti, F Martin and G Maurizi

Monitoring of transcript regulation and protein production related stress responses in Pichia pastoris secreting Fab antibody fragments

Authors: Brigitte Gasser, Michael Maurer, Michael Sauer, Markku Saloheimo, Jari Rautio, Merja Penttila and Diethard Mattanovich

Human Prolactin (hPRL) and Growth Hormone (hGH) distinct behavior under bacteriophage lambda P_L promoter control

Authors: Carlos RJ Soares, Eric KM Ueda, Tais L Oliveira, Susana R Heller and Paolo Bartolini

Design of transcriptional fusions of stress sensitive promoters and GFP to monitor the overburden of E.coli hosts during recombinant protein production

Authors: Sabine Nemecek, Karoline Marisch, Renata Juric and Karl Bayer

Nonclassical inclusion bodies in Escherichia coli

Authors: Špela Peternel, Marjan Bele, Vladka Gaberc-Porekar and Viktor Menart

An optimized method to produce halophilic proteins in Escherichia coli

Authors: Julia Esclapez, Ma José Bonete, Mónica Camacho, Carmen Pire, Juan Ferrer, Vanesa Bautista, Rosa Ma Martínez-Espinosa, Basilio Zafrilla, Francisco Pérez-Pomares and Susana Díaz

Antisense RNA based control of detrimental factors for recombinant gene expression in Escherichia coli – down-regulation of RNase E

Authors: Christian Kemmer and Peter Neubauer

Point mutation of serine 179 in the human Prolactin (PRL) affects recombinant protein expression, folding and secretion, abolishes PRL nickel (II)-binding and increases heparin binding capacities

Authors: Eric Ueda, Carlos Soares, Ameae Walker and Paolo Bartolini

Performance of beta-galactosidase inclusion bodies in enzymatic bioprocesses

Authors: Elena García-Fruitós, Anna Arís and Antonio Villaverde

RNA Interference mediated knockdown of genes in order to increase protein production using the baculovirus expression system

Authors: Colin Hebert, Eun Jeong Kim, Shannon F Kramer, James J Valdes and William E Bentley

DnaK-J are limiting for proper recombinant protein folding only at low production rates and when the physiological heat-shock stress response is not triggered

Authors: Mónica Martínez-Alonso, Andrea Vera, Elena García-Fruitós, Núria González-Montalbán, Anna Arís and Antonio Villaverde

Kinetics of aggregation and structural properties of proteins in inclusion bodies studied by Fourier transform infrared spectroscopy

Authors: Antonino Natalello, Diletta Ami, Pietro Gatti-Lafranconi, Ario de Marco, Marina Lotti and Silvia Maria Doglia

Cellular toxicity triggered by bacterial inclusion bodies

Authors: Nuria González-Montalbán, Antonio Villaverde and Anna Aris

Monitoring the Dynamics of Transcription and Translation Within the Time Course of Recombinant E. coli Cultivations

Authors: Karin Dürrschmid, Wolfgang Schmidt-Heck, Thomas Hrebicek, Helga Reischer, Norbert Auer, Gerd Margreiter, Reinhard Guthke, Andreas Rizzi and Karl Bayer

A sensor of the Unfolded Protein Response to study the stress induced in Yarrowia lipolytica strains by the production of heterologous proteins

Authors: Catherine Madzak and Jean-Marie Beckerich

Transcriptional analysis of the recA gene of Streptococcus thermophilus

RecA is a highly conserved prokaryotic protein that not only plays several important roles connected to DNA metabolism but also affects the cell response to various stress conditions. While RecA is highly cons...

Authors: Gabriele Giliberti, Loredana Baccigalupi, Angelina Cordone, Ezio Ricca and Maurilio De Felice

The evaluation of the factors that cause aggregation during recombinant expression in E. coli is simplified by the employment of an aggregation-sensitive reporter

The yields of soluble recombinant proteins expressed in bacteria are often low due to the tendency of the heterologous proteins to form aggregates. Therefore, aggregation reporters have been envisaged to simpl...

Authors: Tina Schultz, Lucia Martinez and Ario de Marco

Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approach

In the interest of generating large amounts of recombinant protein, inducible systems have been studied to maximize both the growth of the culture and the production of foreign proteins. Even though thermo-ind...

Authors: Marc G Aucoin, Virginie McMurray-Beaulieu, Frédéric Poulin, Eric B Boivin, Jingkui Chen, Francisc M Ardelean, Mathieu Cloutier, Young J Choi, Carlos B Miguez and Mario Jolicoeur

The chaperone DnaK controls the fractioning of functional protein between soluble and insoluble cell fractions in inclusion body-forming cells

The molecular mechanics of inclusion body formation is still far from being completely understood, specially regarding the occurrence of properly folded, protein species that exhibit natural biological activit...

Authors: Nuria González-Montalbán, Elena García-Fruitós, Salvador Ventura, Anna Arís and Antonio Villaverde

Metabolic engineering of microbes for oligosaccharide and polysaccharide synthesis

Metabolic engineering has recently been embraced as an effective tool for developing whole-cell biocatalysts for oligosaccharide and polysaccharide synthesis. Microbial catalysts now provide a practical means ...

Authors: Anne Ruffing and Rachel Ruizhen Chen

A general method for selection of riboflavin-overproducing food grade micro-organisms

This study describes a strategy to select and isolate spontaneous riboflavin-overproducing strains of Lactobacillus (Lb.) plantarum, Leuconostoc (Lc.) mesenteroides and Propionibacterium (P.) freudenreichii.

Authors: Catherine M Burgess, Eddy J Smid, Ger Rutten and Douwe van Sinderen

Live bacterial vaccines – a review and identification of potential hazards

The use of live bacteria to induce an immune response to itself or to a carried vaccine component is an attractive vaccine strategy. Advantages of live bacterial vaccines include their mimicry of a natural inf...

Authors: Ann Detmer and Jacob Glenting

Surface display of proteins by Gram-negative bacterial autotransporters

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful technique with many biotechnological and medical applications. Autotransporters have recently emerged as a good to...

Authors: Nancy Rutherford and Michael Mourez

Miniature bioreactors: current practices and future opportunities

This review focuses on the emerging field of miniature bioreactors (MBRs), and examines the way in which they are used to speed up many areas of bioprocessing. MBRs aim to achieve this acceleration as a result...

Authors: Jonathan I Betts and Frank Baganz

Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae

Industrial biotechnology employs the controlled use of microorganisms for the production of synthetic chemicals or simple biomass that can further be used in a diverse array of applications that span the pharm...

Authors: Joseph A Chemler, Yajun Yan and Mattheos AG Koffas

Improvement of bioprocess monitoring: development of novel concepts

The advancement of bioprocess monitoring will play a crucial role to meet the future requirements of bioprocess technology. Major issues are the acceleration of process development to reduce the time to the ma...

Authors: Franz Clementschitsch and Karl Bayer

Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains

Fermentation of lignocellulosic biomass is an attractive alternative for the production of bioethanol. Traditionally, the yeast Saccharomyces cerevisiae is used in industrial ethanol fermentations. However, S. ce...

Authors: Kaisa Karhumaa, Beate Wiedemann, Bärbel Hahn-Hägerdal, Eckhard Boles and Marie-F Gorwa-Grauslund

Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for heterologous protein production. The use of different phenotypes under PAOX promoter, other alternative promot...

Authors: Oriol Cos, Ramón Ramón, José Luis Montesinos and Francisco Valero

Performances and microbial features of an aerobic packed-bed biofilm reactor developed to post-treat an olive mill effluent from an anaerobic GAC reactor

Olive mill wastewater (OMW) is the aqueous effluent of olive oil producing processes. Given its high COD and content of phenols, it has to be decontaminated before being discharged. Anaerobic digestion is one ...

Authors: Lorenzo Bertin, Maria Chiara Colao, Maurizio Ruzzi, Leonardo Marchetti and Fabio Fava