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  1. Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty. The lack of a published genome sequence re...

    Authors: Michael Sauer, Paola Branduardi, Brigitte Gasser, Minoska Valli, Michael Maurer, Danilo Porro and Diethard Mattanovich

    Citation: Microbial Cell Factories 2004 3:17

    Content type: Research

    Published on:

  2. Antibody fragments are molecules widely used for diagnosis and therapy. A large amount of protein is frequently required for such applications. New approaches using folding reporter enzymes have recently been ...

    Authors: Pascal Philibert and Pierre Martineau

    Citation: Microbial Cell Factories 2004 3:16

    Content type: Research

    Published on:

  3. Sugar binding proteins and binders of intermediate sugar metabolites derived from microbes are increasingly being used as reagents in new and expanding areas of biotechnology. The fixation of carbon dioxide at...

    Authors: Sourav Mahato, Debojyoti De, Debajyoti Dutta, Moloy Kundu, Sumana Bhattacharya, Marc T Schiavone and Sanjoy K Bhattacharya

    Citation: Microbial Cell Factories 2004 3:7

    Content type: Review

    Published on:

  4. The genome sequence analysis of Thermus thermophilus HB27, a microorganism with high biotechnological potential, has recently been published. In that report, the chromosomal and the megaplasmid sequence were comp...

    Authors: Efthimia E Lioliou, Anastasia A Pantazaki and Dimitrios A Kyriakidis

    Citation: Microbial Cell Factories 2004 3:5

    Content type: Commentary

    Published on:

  5. The proper functioning of extracytoplasmic proteins requires their export to, and productive folding in, the correct cellular compartment. All proteins in Escherichia coli are initially synthesized in the cytopla...

    Authors: Marika Miot and Jean-Michel Betton

    Citation: Microbial Cell Factories 2004 3:4

    Content type: Review

    Published on:

  6. Bacillus cereus constitutes a significant cause of acute food poisoning in humans. Despite the recent development of different detection methods, new effective control measures and better diagnostic tools are req...

    Authors: Magdalena Gabig-Ciminska, Heiko Andresen, Joerg Albers, Rainer Hintsche and Sven-Olof Enfors

    Citation: Microbial Cell Factories 2004 3:2

    Content type: Research

    Published on:

  7. A protein quality control system, consisting of molecular chaperones and proteases, controls the folding status of proteins and prevents the aggregation of misfolded proteins by either refolding or degrading a...

    Authors: Jimena Weibezahn, Bernd Bukau and Axel Mogk

    Citation: Microbial Cell Factories 2004 3:1

    Content type: Review

    Published on:

  8. A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut+ strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. A recombinant fusion ...

    Authors: Mehmedalija Jahic, Fredrik Wallberg, Monika Bollok, Percival Garcia and Sven-Olof Enfors

    Citation: Microbial Cell Factories 2003 2:6

    Content type: Research

    Published on:

  9. The bioavailability of iron is quite low since it is usually present as insoluble complexes. To solve the bioavailability problem microorganisms have developed highly efficient iron-scavenging systems based on...

    Authors: Francisco J Flores, Javier Rincón and Juan F Martín

    Citation: Microbial Cell Factories 2003 2:5

    Content type: Research

    Published on:

  10. A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were...

    Authors: Jari Rautio, Kim Bundvig Barken, Juhani Lahdenperä, Antje Breitenstein, Søren Molin and Peter Neubauer

    Citation: Microbial Cell Factories 2003 2:4

    Content type: Research

    Published on:

  11. In contrast to bioreactors the metabolites within the microbial cells are converted in an impure atmosphere, yet the productivity seems to be well regulated and not affected by changes in operation variables. ...

    Authors: Sumana Bhattacharya, Subhra Chakrabarti, Amiya Nayak and Sanjoy K Bhattacharya

    Citation: Microbial Cell Factories 2003 2:3

    Content type: Review

    Published on:

  12. Use of lactose-rich concentrates from dairy processes for the induction of recombinant gene's expression has not received much attention although they are interesting low cost substrates for production of reco...

    Authors: Mikko I Viitanen, Antti Vasala, Peter Neubauer and Tapani Alatossava

    Citation: Microbial Cell Factories 2003 2:2

    Content type: Research

    Published on:

  13. In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody...

    Authors: Vivi Joosten, Christien Lokman, Cees AMJJ van den Hondel and Peter J Punt

    Citation: Microbial Cell Factories 2003 2:1

    Content type: Review

    Published on:

  14. The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans. CGTases are produced by a variety o...

    Authors: Adriana M Rosso, Susana A Ferrarotti, Norberto Krymkiewicz and Bclara Nudel

    Citation: Microbial Cell Factories 2002 1:3

    Content type: Research

    Published on:

  15. Applications of bacteriocins as food preservatives have been so far limited, principally because of their low antimicrobial activity in foods. Nisin is the only bacteriocin of significant use, but applications...

    Authors: Luca Martirani, Mario Varcamonti, Gino Naclerio and Maurilio De Felice

    Citation: Microbial Cell Factories 2002 1:1

    Content type: Research

    Published on:

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