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  1. Content type: Review

    This article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. Biofilm formation is a natural process where microbial cells attach to the s...

    Authors: Nasib Qureshi, Bassam A Annous, Thaddeus C Ezeji, Patrick Karcher and Ian S Maddox

    Citation: Microbial Cell Factories 2005 4:24

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  2. Content type: Review

    Reduced protein stability in vivo is a prerequisite to aggregation. While this is merely a nuisance factor in recombinant protein production, it holds a serious impact for man. This review focuses on specific app...

    Authors: Zoya Ignatova

    Citation: Microbial Cell Factories 2005 4:23

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  3. Content type: Research

    The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets huma...

    Authors: Konrad Büssow, Christoph Scheich, Volker Sievert, Ulrich Harttig, Jörg Schultz, Bernd Simon, Peer Bork, Hans Lehrach and Udo Heinemann

    Citation: Microbial Cell Factories 2005 4:21

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  4. Content type: Research

    It has been shown that proteins fused to ubiquitin undergo greater expression in E. coli and are easier to purify and renaturate than nonhybrid foreign proteins. However, there is no commercial source of large qu...

    Authors: Anna Wojtowicz, Anna Mazurkiewicz-Pisarek, Grazyna Plucienniczak, Diana Mikiewicz-Sygula, Luiza Chojnacka, Natalia Lukasiewicz and Andrzej Plucienniczak

    Citation: Microbial Cell Factories 2005 4:17

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  5. Content type: Research

    The nisin-controlled gene expression system NICE of Lactococcus lactis is one of the most widely used expression systems in Gram-positive bacteria. Despite its widespread use, no optimization of the culture condi...

    Authors: Igor Mierau, Kees Olieman, James Mond and Eddy J Smid

    Citation: Microbial Cell Factories 2005 4:16

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  6. Content type: Research

    The NI sin-C ontrolled gene E xpression system NICE of Lactococcus lactis is one of the most widespread used expression systems of Gram-positive bacteria. It is used in more than 100 laboratories for laboratory-s...

    Authors: Igor Mierau, Peter Leij, Iris van Swam, Barry Blommestein, Esther Floris, James Mond and Eddy J Smid

    Citation: Microbial Cell Factories 2005 4:15

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  7. Content type: Review

    Microorganisms are used in large-scale heap or tank aeration processes for the commercial extraction of a variety of metals from their ores or concentrates. These include copper, cobalt, gold and, in the past,...

    Authors: Douglas E Rawlings

    Citation: Microbial Cell Factories 2005 4:13

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  8. Content type: Review

    Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GF...

    Authors: Wei Wen Su

    Citation: Microbial Cell Factories 2005 4:12

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  9. Content type: Editorial

    As in other Biotechnological fields, the microbial production of recombinant proteins and other biomolecules can be approached from multiple angles through the help of diverse technologies of increasing comple...

    Authors: Antonio Villaverde

    Citation: Microbial Cell Factories 2005 4:10

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  10. Content type: Research

    Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the ...

    Authors: Jaakko Soini, Christina Falschlehner, Christina Mayer, Daniela Böhm, Stefan Weinel, Johanna Panula, Antti Vasala and Peter Neubauer

    Citation: Microbial Cell Factories 2005 4:9

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  11. Content type: Review

    Actinomycetes (Actinobacteria) are highly attractive as cell factories or bioreactors for applications in industrial, agricultural, environmental, and pharmaceutical fields. Genome sequencing of several species o...

    Authors: Nobutaka Nakashima, Yasuo Mitani and Tomohiro Tamura

    Citation: Microbial Cell Factories 2005 4:7

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  12. Content type: Research

    The permanently impaired protein folding during recombinant protein production resembles the stress encountered at extreme temperatures, under which condition the putative holding chaperones, IbpA/IbpB, play a...

    Authors: Ha LeThanh, Peter Neubauer and Frank Hoffmann

    Citation: Microbial Cell Factories 2005 4:6

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  13. Content type: Research

    Carbon dioxide fixation bioprocess in reactors necessitates recycling of D-ribulose1,5-bisphosphate (RuBP) for continuous operation. A radically new close loop of RuBP regenerating reactor design has been prop...

    Authors: Debojyoti De, Debajyoti Dutta, Moloy Kundu, Sourav Mahato, Marc T Schiavone, Surabhi Chaudhuri, Ashok Giri, Vidya Gupta and Sanjoy K Bhattacharya

    Citation: Microbial Cell Factories 2005 4:5

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  14. Content type: Research

    Hydrocarbon-degrading biofilms in the treatment of contaminated groundwaters have received increasing attention due to the role played in the so-called "biobarriers". These are bioremediation systems in which ...

    Authors: Alessandra Di Lorenzo, Mario Varcamonti, Palma Parascandola, Rodolfo Vignola, Adriano Bernardi, Pasquale Sacceddu, Raffaello Sisto and Elisabetta de Alteriis

    Citation: Microbial Cell Factories 2005 4:4

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  15. Content type: Review

    Lactococcus lactis, the model lactic acid bacterium (LAB), is a food grade and well-characterized Gram positive bacterium. It is a good candidate for heterologous protein delivery in foodstuff or in the digestive...

    Authors: Yves Le Loir, Vasco Azevedo, Sergio C Oliveira, Daniela A Freitas, Anderson Miyoshi, Luis G Bermúdez-Humarán, Sébastien Nouaille, Luciana A Ribeiro, Sophie Leclercq, Jane E Gabriel, Valeria D Guimaraes, Maricê N Oliveira, Cathy Charlier, Michel Gautier and Philippe Langella

    Citation: Microbial Cell Factories 2005 4:2

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  16. Content type: Research

    Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty. The lack of a published genome sequence re...

    Authors: Michael Sauer, Paola Branduardi, Brigitte Gasser, Minoska Valli, Michael Maurer, Danilo Porro and Diethard Mattanovich

    Citation: Microbial Cell Factories 2004 3:17

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  17. Content type: Research

    Antibody fragments are molecules widely used for diagnosis and therapy. A large amount of protein is frequently required for such applications. New approaches using folding reporter enzymes have recently been ...

    Authors: Pascal Philibert and Pierre Martineau

    Citation: Microbial Cell Factories 2004 3:16

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  18. Content type: Review

    Sugar binding proteins and binders of intermediate sugar metabolites derived from microbes are increasingly being used as reagents in new and expanding areas of biotechnology. The fixation of carbon dioxide at...

    Authors: Sourav Mahato, Debojyoti De, Debajyoti Dutta, Moloy Kundu, Sumana Bhattacharya, Marc T Schiavone and Sanjoy K Bhattacharya

    Citation: Microbial Cell Factories 2004 3:7

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  19. Content type: Commentary

    The genome sequence analysis of Thermus thermophilus HB27, a microorganism with high biotechnological potential, has recently been published. In that report, the chromosomal and the megaplasmid sequence were comp...

    Authors: Efthimia E Lioliou, Anastasia A Pantazaki and Dimitrios A Kyriakidis

    Citation: Microbial Cell Factories 2004 3:5

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  20. Content type: Review

    The proper functioning of extracytoplasmic proteins requires their export to, and productive folding in, the correct cellular compartment. All proteins in Escherichia coli are initially synthesized in the cytopla...

    Authors: Marika Miot and Jean-Michel Betton

    Citation: Microbial Cell Factories 2004 3:4

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  21. Content type: Research

    Bacillus cereus constitutes a significant cause of acute food poisoning in humans. Despite the recent development of different detection methods, new effective control measures and better diagnostic tools are req...

    Authors: Magdalena Gabig-Ciminska, Heiko Andresen, Joerg Albers, Rainer Hintsche and Sven-Olof Enfors

    Citation: Microbial Cell Factories 2004 3:2

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  22. Content type: Review

    A protein quality control system, consisting of molecular chaperones and proteases, controls the folding status of proteins and prevents the aggregation of misfolded proteins by either refolding or degrading a...

    Authors: Jimena Weibezahn, Bernd Bukau and Axel Mogk

    Citation: Microbial Cell Factories 2004 3:1

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  23. Content type: Research

    A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut+ strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. A recombinant fusion ...

    Authors: Mehmedalija Jahic, Fredrik Wallberg, Monika Bollok, Percival Garcia and Sven-Olof Enfors

    Citation: Microbial Cell Factories 2003 2:6

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  24. Content type: Research

    The bioavailability of iron is quite low since it is usually present as insoluble complexes. To solve the bioavailability problem microorganisms have developed highly efficient iron-scavenging systems based on...

    Authors: Francisco J Flores, Javier Rincón and Juan F Martín

    Citation: Microbial Cell Factories 2003 2:5

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  25. Content type: Research

    A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were...

    Authors: Jari Rautio, Kim Bundvig Barken, Juhani Lahdenperä, Antje Breitenstein, Søren Molin and Peter Neubauer

    Citation: Microbial Cell Factories 2003 2:4

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  26. Content type: Review

    In contrast to bioreactors the metabolites within the microbial cells are converted in an impure atmosphere, yet the productivity seems to be well regulated and not affected by changes in operation variables. ...

    Authors: Sumana Bhattacharya, Subhra Chakrabarti, Amiya Nayak and Sanjoy K Bhattacharya

    Citation: Microbial Cell Factories 2003 2:3

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  27. Content type: Research

    Use of lactose-rich concentrates from dairy processes for the induction of recombinant gene's expression has not received much attention although they are interesting low cost substrates for production of reco...

    Authors: Mikko I Viitanen, Antti Vasala, Peter Neubauer and Tapani Alatossava

    Citation: Microbial Cell Factories 2003 2:2

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  28. Content type: Review

    In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody...

    Authors: Vivi Joosten, Christien Lokman, Cees AMJJ van den Hondel and Peter J Punt

    Citation: Microbial Cell Factories 2003 2:1

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