Volume 5 Supplement 1

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

Meeting abstracts

The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting.

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology.

Barcelona, Spain21-23 September 2006

Concatenated PDF 5S1

Page 3 of 3

Evaluation of the baculovirus and E.coli-expressed non-structural (NS) proteins of bluetongue virus (BTV) as antigen in an indirect or competition ELISA to differentiate infected from vaccinated animals

Authors: Lissette López, Angel Venteo, Miguel Angel Jiménez-Clavero, Juan Luis Carrasco, María José Cano, Elena Soria, Concepción Gómez-Tejedor, Antonio Sanz, Carmen Vela and Paloma Rueda

Citation: Microbial Cell Factories 2006 5(Suppl 1):P59

Content type: Poster Presentation

Published on: 10 October 2006
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Automation for higher throughput in protein expression: visions, facts and fictions

Authors: Marion Mahnke, Jean Marc Schlaeppi, Yann Pouliquen, Louise Barys, Catherine Rolvering, Mario Henke, Rita Schmitz, Sabine Geisse and Frank Kolbinger

Citation: Microbial Cell Factories 2006 5(Suppl 1):P60

Content type: Poster Presentation

Published on: 10 October 2006
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Potentials and limitations of prokaryotic and eukaryotic expression systems for recombinant protein production – a comparative view

Authors: Dethardt Müller, Karl Bayer and Diethard Mattanovich

Citation: Microbial Cell Factories 2006 5(Suppl 1):P61

Content type: Poster Presentation

Published on: 10 October 2006
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Production of human α₁ proteinase inhibitor from Aspergillus niger

Authors: Liat Chill, Loc B Trinh, Elena Karnaukhova, Yakir Ophir, Basil Golding and Joseph Shiloach

Citation: Microbial Cell Factories 2006 5(Suppl 1):P62

Content type: Poster Presentation

Published on: 10 October 2006
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Kluyveromyces lactis SSO1 and SEB1 genes are functional in Saccharomyces cerevisiae and enhance production of secreted proteins when overexpressed

Authors: Jaana H Toikkanen, Lena Sundqvist and Sirkka Keränen

Citation: Microbial Cell Factories 2006 5(Suppl 1):P63

Content type: Poster Presentation

Published on: 10 October 2006
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Antibody production by a protease-deficient strain of methylotrophic yeast, Ogataea minuta

Authors: Kousuke Kuroda, Yoshinori Kitagawa, Kazuo Kobayashi, Haruhiko Tsumura, Toshihiro Komeda, Yasunori Chiba and Yoshihumi Jigami

Citation: Microbial Cell Factories 2006 5(Suppl 1):P64

Content type: Poster Presentation

Published on: 10 October 2006
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Heterologous expression of isotopically labeled Trichoderma reesei tyrosinase 2 in Pichia pastoris

Authors: Ann Westerholm-Parvinen, Maija-Liisa Mattinen, Emilia Selinheimo and Markku Saloheimo

Citation: Microbial Cell Factories 2006 5(Suppl 1):P65

Content type: Poster Presentation

Published on: 10 October 2006
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Secretion of a hybrid K. lactis-A. niger β-galactosidase

Authors: Ángel Pereira, Rafael Fernández, María Esperanza Cerdán, María Isabel González Siso and Manuel Becerra

Citation: Microbial Cell Factories 2006 5(Suppl 1):P66

Content type: Poster Presentation

Published on: 10 October 2006
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The first auxotrophic mutant of Zygosaccharomyces bailii for recombinant productions: a road to practical applications

Authors: Paola Branduardi, Laura Dato, Luca Riboldi and Danilo Porro

Citation: Microbial Cell Factories 2006 5(Suppl 1):P67

Content type: Poster Presentation

Published on: 10 October 2006
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Direct and indirect approaches for the improvement of heterologous proteins secretion levels in Zygosaccharomyces bailii

Authors: Luca Riboldi, Danilo Porro, Laura Dato and Paola Branduardi

Citation: Microbial Cell Factories 2006 5(Suppl 1):P68

Content type: Poster Presentation

Published on: 10 October 2006
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Disruption of the GAS1 gene of Pichia pastoris confers a supersecretory phenotype for Rhizopus oryzae lipase, but not for human trypsinogen

Authors: Hans Marx, Michael Sauer, David Resina, Marina Vai, Danilo Porro, Francisco Valero, Pau Ferrer and Diethard Mattanovich

Citation: Microbial Cell Factories 2006 5(Suppl 1):P69

Content type: Poster Presentation

Published on: 10 October 2006
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Genomic and proteomics approaches for vaccine development in Pasteurella multocida

Authors: Keith Al-Hasani, Victoria McCarl, Stephen Bottomley, Ben Adler and John Boyce

Citation: Microbial Cell Factories 2006 5(Suppl 1):P71

Content type: Poster Presentation

Published on: 10 October 2006
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Stabilization of heterologous transcripts with hrpA, mRNA of a type III secretion system component

Authors: Elina Hienonen, Martin Romantschuk and Suvi Taira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P72

Content type: Poster Presentation

Published on: 10 October 2006
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Over-expression and single-step purification of human IFN_α8 and human IFN_α2b reveals the highest antiviral activity of human IFN_α8

Authors: Julio César Sánchez García, Alejandro Miranda Ariza, Alexis Musacchio Lassa, Luis Javier González and Vladimir Besada Perez

Citation: Microbial Cell Factories 2006 5(Suppl 1):P73

Content type: Poster Presentation

Published on: 10 October 2006
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Bacillus megaterium as a recombinant protein production host

Authors: Yang Yang, Marco Malten, Rebekka Biedendieck, Wei Wang, Dieter Jahn and Wolf-Dieter Deckwer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P74

Content type: Poster Presentation

Published on: 10 October 2006
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Design and production of multi-bioactive recombinant elastin-like polymer: Mimicking the extracellular matrix

Authors: Alessandra Girotti, F Javier Arias, Ana M Testera and J Carlos Rodriguez-Cabello

Citation: Microbial Cell Factories 2006 5(Suppl 1):P75

Content type: Poster Presentation

Published on: 10 October 2006
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N-terminally acetylated tropomyosin generated in E. coli by coexpression of the S. cerevisiae NatB acetylation complex shows functional properties in vitro

Authors: Robin Maytum and Manfred Konrad

Citation: Microbial Cell Factories 2006 5(Suppl 1):P76

Content type: Poster Presentation

Published on: 10 October 2006
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Use of the tetA-promoter in fed-batch cultivations: Repeated supply of anhydrotetracycline is necessary for production of tetrameric collagen prolyl 4-hydroxylase in Escherichia coli

Authors: A Neubauer, J Soini, M Bollok, M Zenker, J Sandquist, J Myllyharju and P Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P77

Content type: Poster Presentation

Published on: 10 October 2006
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Monitoring protein expression levels in E. coli using a high throughput approach

Authors: Régis Cébe and Martin Geiser

Citation: Microbial Cell Factories 2006 5(Suppl 1):P78

Content type: Poster Presentation

Published on: 10 October 2006
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Recombinant protein production in cell-free systems: strategies for improving yield and functionality

Authors: Jan Strey, Michael Gerrits, Stefan Kubick, Helmut Merk, Uritza von Groll, Frank Schäfer and Wolfgang Stiege

Citation: Microbial Cell Factories 2006 5(Suppl 1):P79

Content type: Poster Presentation

Published on: 10 October 2006
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A regulatory acceptable alternative to E. coli: high yield recombinant protein production using the Lactococcus lactis P170 expression system combined with "Reverse electro enhanced dialysis" (REED) for lactate control

Authors: Søren M Madsen, Astrid Vrang, Lars H Pedersen, Sean A MacDonald, Jens-Ulrik Rype and Arvid Garde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P80

Content type: Poster Presentation

Published on: 10 October 2006
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A simple emergency procedure to be used if biotechnological protein production is endangered by bacteriophage infection of Escherichia coli cultures: effective inhibition of bacteriophage lytic development in infected cultures by removing a carbon source from the medium

Authors: Grzegorz Wegrzyn, Marcin Los and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P81

Content type: Poster Presentation

Published on: 10 October 2006
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A counter-selectable marker for Bacillus

Authors: Michael D Rasmussen, Jan Martinussen, Els Marie Celine Defoor and Gitte Bak Poulsen

Citation: Microbial Cell Factories 2006 5(Suppl 1):P82

Content type: Poster Presentation

Published on: 10 October 2006
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The expression of truncated form of CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) from genetically modified plant in Escherichia coli

Authors: Stanislav Stuchlík, Satheesh Natarajan and Ján Turña

Citation: Microbial Cell Factories 2006 5(Suppl 1):P83

Content type: Poster Presentation

Published on: 10 October 2006
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Recombinant lipase immobilised in the cell wall of Bacillus halodurans Alk 36 exploiting the FliC protein

Authors: Michael Crampton, Erika du Plessis, Santosh Ramchuran, Eldie Berger and Maureen Louw

Citation: Microbial Cell Factories 2006 5(Suppl 1):P84

Content type: Poster Presentation

Published on: 10 October 2006
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Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli

Authors: Luis Vidal, Josep López-Santín, Glòria Caminal and Pau Ferrer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P85

Content type: Poster Presentation

Published on: 10 October 2006
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Development of extracellular production system of recombinant proteins in recombinant Escherichia coli

Authors: Jong Hyun Choi, Zhi Gang Qian and Sang Yup Lee

Citation: Microbial Cell Factories 2006 5(Suppl 1):P86

Content type: Poster Presentation

Published on: 10 October 2006
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ColE1 derived RNA I as a key molecule in a novel antibiotic free plasmid addiction system

Authors: Irene Pfaffenzeller, Gerald Striedner, Karl Bayer and Reingard Grabherr

Citation: Microbial Cell Factories 2006 5(Suppl 1):P87

Content type: Poster Presentation

Published on: 10 October 2006
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Expression of trehalose-6-phosphate synthase gene from Arabidopsis thaliana in transgenic tobacco: a strategy to increase temperature stress tolerance

Authors: André de Almeida, Enrique Villalobos, Susana Araújo, Luís A Cardoso, Dulce Santos, José M Torné and Pedro S Fevereiro

Citation: Microbial Cell Factories 2006 5(Suppl 1):P88

Content type: Poster Presentation

Published on: 10 October 2006
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Expression of functional recombinant rabies virus glycoprotein in Drosophila melanogaster S2 cells

Authors: Adriana Y Yokomizo, Soraia AC Jorge, Renato M Astray, Mariza AG Santos, Irene Fernandes, Orlando G Ribeiro, Denise SPQ Horton, Aldo Tonso and Carlos A Pereira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P89

Content type: Poster Presentation

Published on: 10 October 2006
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Effects of overexpression of X-box binding protein 1 on recombinant protein production in mammalian cells

Authors: Sebastian Ku, Grace Chong, Maybelline Giam, Miranda GS Yap and Sheng-Hao Chao

Citation: Microbial Cell Factories 2006 5(Suppl 1):P90

Content type: Poster Presentation

Published on: 10 October 2006
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Analysis and characterization of different preparations of recombinant human follicle stimulating hormone (hFSH) and of its subunits

Authors: Maria Teresa CP Ribela, Renan F Loureiro, João E Oliveira, Cristiane M Carvalho, Cibele N Peroni and Paolo Bartolini

Citation: Microbial Cell Factories 2006 5(Suppl 1):P91

Content type: Poster Presentation

Published on: 10 October 2006
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Characterization of Medicago truncatula cell suspension cultures producing valuable recombinant proteins

Authors: Guadalupe Cabral, Ana Sofia Pires, Pablo Gonzalez-Melendi and Rita Abranches

Citation: Microbial Cell Factories 2006 5(Suppl 1):P92

Content type: Poster Presentation

Published on: 10 October 2006
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A novel in vitro translation system based on insect cells

Authors: Stefan Kubick, Helmut Merk, Michael Gerrits, Jan Strey, Uritza von Groll, Frank Schäfer and Wolfgang Stiege

Citation: Microbial Cell Factories 2006 5(Suppl 1):P93

Content type: Poster Presentation

Published on: 10 October 2006
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Purification of a chimeric virus-like particle from a complex culture medium

Authors: Luísa Pedro and Guilherme NM Ferreira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P95

Content type: Poster Presentation

Published on: 10 October 2006
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Identifying key signatures of highly productive CHO cells from transcriptome and proteome profiles

Authors: Arleen Sanny, Yee Jiun Kok, Robin Philip, Song Hui Chuah, Sze Wai Ng, Kher Shing Tan, Lee Yih Yean, Kathy Wong, Hu Weishou, Miranda Yap and Peter Morin Nissom

Citation: Microbial Cell Factories 2006 5(Suppl 1):P96

Content type: Poster Presentation

Published on: 10 October 2006
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Kinetics of aggregation and structural properties of proteins in inclusion bodies studied by Fourier transform infrared spectroscopy

Authors: Antonino Natalello, Diletta Ami, Pietro Gatti-Lafranconi, Ario de Marco, Marina Lotti and Silvia Maria Doglia

Citation: Microbial Cell Factories 2006 5(Suppl 1):P97

Content type: Poster Presentation

Published on: 10 October 2006
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Production and purification of high molecular weight oligomers of Yersinia pestis F1 capsular antigen released by high cell density culture of recombinant Escherichia coli cells carrying the caf1 operon

Authors: Tzvi Holtzman, Yinon Levy, Dino Marcus, Yehuda Flashner, Emanuelle Mamroud, Sara Cohen and Rephael Fass

Citation: Microbial Cell Factories 2006 5(Suppl 1):P98

Content type: Poster Presentation

Published on: 10 October 2006
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The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

Meeting abstracts

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