Fig. 4
From: Manufacture and evaluation of a HER2-positive breast cancer immunotoxin 4D5Fv-PE25
Detection of HER2 expression in breast cancer cell lines and the evaluation of 4D5Fv-PE25 in vitro and in vivo. A: Detection of HER2 expression in different cell lines. Blue area: mIgG control, red area: Anti-HER2 antibody. B: The killing effect of 4D5Fv-PE25 on different breast cancer cell lines. C: The evaluation of the immunotoxin in the co-culture assay. D: The inhibition effect on the solid tumor of 4D5Fv-PE25. The black arrows represent the injection time point. n = 5 in control group, n = 10 in 4D5Fv-PE25 group. E: Clearance rate of the 4D5Fv-PE25 in mice (n = 6). Data was illustrated as means ± SD, and D was analyzed by two-way ANOVA followed by the Bonferroni test. *P < 0.05, **P < 0.01