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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Manufacture and evaluation of a HER2-positive breast cancer immunotoxin 4D5Fv-PE25

Fig. 2

Growth curve of engineering bacteria and purity test of 4D5Fv-PE25. A: Growth curve of engineering bacteria (n = 3). B: Detection of the effect of removing impure protein by equilibrium buffer in Capto Butyl gel. M: marker, 1–8: 8 kinds of equilibrium buffer (30% saturation ammonium sulfate/chromatography buffer A (v/v)): 10%, 20%, 30%, 40%, 50%, 60%, 70%, 100%. C: Elution effect of different gels and eluent. 1: ethanol-free chromatography buffer A, 2: deionized water, 3: 0.5 M sodium hydroxide, 4: chromatography buffer A containing 1% ethanol (v/v), 5: deionized water, 6: 0.5 M sodium hydroxide, 7: chromatography buffer A containing 5% ethanol (v/v), 8: deionized water, 9: 0.5 M sodium hydroxide, 10: chromatography buffer A containing 10% ethanol (v/v), 11: deionized water, 12: 0.5 M sodium hydroxide. D: Purification effect of Capto S ImpAct and Capto Q. 1: the flowing through protein of Capto S Impact, 2: the binding protein of Capto S Impact, 3: the flowing through protein of Capto Q, 4: the binding protein of Capto Q. E: Purification effect of Capto S ImpAct at different flow rates. 1: 6.0 mm/min, 2: 15.0 mm/min. F: purity SDS-PAGE detection. G: Purity chromatographic detection

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