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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Manufacture and evaluation of a HER2-positive breast cancer immunotoxin 4D5Fv-PE25

Fig. 1

Structural model of the 4D5Fv-PE25. VL and VH: the variable region of trastuzumab, PE25: Pseudomonas exotoxin, KDEL: potent toxin fragment, G: glycine, S: serine, Linker: the last 20 amino acids of human muscle aldolase, characterized by flexibility, easy folding, and no antigenicity. The cartoon was generated with PyMOL software, in which the structure of VL, VH, and PE25 was built based on the X-ray crystal structure of HER2-binding scFv-Fab fusion (Protein Data Bank code: 6ZQK) and Pseudomonas Aeruginosa Exotoxin A (Protein Data Bank code: 6EDG).

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