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Fig. 10 | Microbial Cell Factories

Fig. 10

From: Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046

Fig. 10

Correlation of final broth viscosity and accumulated oxygen transfer of all presented A. vinelandii cultivations. A. vinelandii ATCC9046 was cultivated in modified Burk’s medium with varying concentrations and suppliers of yeast extract as well as different supplements. If not stated otherwise in the legend of this diagram, 3 g/L yeast extract from Roth Batch 1 were used. The standard deviation was calculated for n ≥ 3. Cultivation conditions for microaerobic conditions: 250 mL shake flasks, filling volume VL = 50 mL, temperature T = 29 °C, shaking frequency n = 165 rpm, shaking diameter d0 = 50 mm, initial pH = 7.2, initial pH for AA group 1 = 6.78, initial pH for AA group 2 & 4 and supplementation of aspartate = 6.95. Cultivation conditions for oxygen non-limiting conditions: 250 mL shake flasks, filling volume VL = 10 mL, temperature T = 29 °C, shaking frequency n = 350 rpm, shaking diameter d0 = 50 mm, initial pH = 7.2

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