Fig. 5.
1H-NMR spectra of peaks N°2 and N°5 conformers measured at 600 MHz. Spectra from basic, acidic species and control samples show the conformational and temporal stability of the species. The samples were exhaustively co-dialysed before analysis. Samples conditions: pH 5.5 and 25 ℃, sample concentration ~ 50 µM. A Spectra of peak N°2 from BL21 (purple) and W3110 (blue). C Peak N°5 from BL21 (grey) and W3110 (green) representing analysis of the same species showing reproducible conformational state as a control and spectra showing the clear difference in the fingerprint of peak N°2 and N°5 conformers from W3110 (B) and BL21 (D). The box represents the expansion of the high field methyl aromatic fingerprint region