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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study

Fig. 3

scFvM purification via two steps chromatography. A Representative Coomassie blue-stained gel of the scFvM produced with BL21 and purified via IMAC. The samples analysed are the total protein sample loaded (L), column flow-though (FT), eluates (E) from either the periplasm or the culture supernatant and the purified protein after dialysis (D) in non-reducing condition. Ladder (Mark12™ Unstained Standard) on the left in kDa. B Representative MonoS normalised chromatograms of scFvM purified from the culture supernatant. Peaks are labelled with numbers ranging from 1 to 7. C Relative abundance of the CEX peaks in BL21 and W3110. BL21 strain is represented in black and W3110 in grey. D Representative Coomassie blue-stained gel of a CEX run in non-reducing condition on the scFvM produced with the BL21 strain. peak N°2 elution fractions are in the continued box while peak N°5 ones are in the dotted box

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