Skip to main content
Fig. 2 | Microbial Cell Factories

Fig. 2

From: Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study

Fig. 2

Lysates of BL21 and W3110 from 5 L fermenters when expressing OmpA-scFvM. Suspension and supernatant samples of BL21 and W3110 expressing OmpA-scFvM were recovered at different time points: T0, T4, T7 and T10 for SDS-PAGE analysis in reducing condition. Representative Coomassie blue-stained gel of the total titer (TT, comprising soluble and insoluble proteins), total soluble (TS, comprising intracellular and extracellular soluble proteins) and supernatant samples (SN, only proteins located in the supernatant) and scFvM reference protein.Ladder (Mark12™ Unstained Standard) on the left in kDa. The same volume of sample was treated and loaded for comparison. OD of the samples: BL21: T0 (167); T4 (229); T7 (231); T10 (212) and W3110: T0 (165); T4 (217); T7 (236); T10(272)

Back to article page