Fig. 1

Soluble scFvM production in BL21 and W3110 in 10 mL and 5 L scale fermentations. A Specific soluble product titer in BL21 and W3110 in 10 mL and 5 L bioreactors expressed in mg/OD. To calculate these values, the 7 h post-induction soluble scFvM titer is divided by the OD₅₅₀ of the culture at that time in both scale bioreactors. 10 mL fermentations specific soluble product formation values are the average of four replicates, error bars have been added to the figure. B Soluble titer variation of the scFvM in BL21 and W3110 strains at different time points [0 h (T0), 4 h (T4), 7 h (T7) and 10 h (T10) post-induction] in 5 L fermentations expressed in g/L. In both graphs, BL21 strain is represented in black and W3110 in grey. Soluble scFvM product was determined by immunoassay from suspension samples. 5 L bioreactors were run two times for each strain with slight variations in temperature, inductor concentration and pH resulting in comparable profiles for OD₅₅₀ and titer