Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source

Fig. 1

Schematic representation of protein production systems using Trichoderma reesei. a In T. reesei strains, (i) cellulose-derived sugars (such as cellobiose) released by cellobiohydrolase (CBH)1 and CBH2 induce the production of large amounts of cellulases and hemicellulases. (ii) When glucose is used as the carbon source, the production of cellulase and hemicellulase is suppressed by carbon catabolite repression (CCR). b In inducer-free T. reesei E1AB1-XA3 strain, with release from CCR and modification of the transcription factors, (i) it is possible to produce cellulases and hemicellulases using only glucose as the carbon source. As a strategy for heterologous protein production, (ii) the most abundant native cellulase, CBH1, is replaced with the target protein, and (iii) high purity and high production are possible by replacing CBH2 with other CAZymes

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us