Current genetic strategies to investigate gene functions in Trichoderma reesei

Table 1 Selection markers used in T. reesei

Gene	Encoded protein	Source	Refs.
Auxotrophic markers
pyr4	orotidine-5-monophosphate decarboxylase	Trichoderma ressei	[32]
pyr2	orotate phosphoribosyl transferase	Trichoderma ressei	[33]
asl1	argininosuccinate lyase	Trichoderma ressei	[34]
ade2	phosphoribosylaminoimidazole carboxylase	Trichoderma ressei	[33]
suc1	invertase	Aspergillus niger	[24]
hxk1	hexokinase	Trichoderma ressei	[25]
hem8	ferrochelatase	Trichoderma ressei	[26]
Antibiotic markers
hph	hygromycin B phosphotransferase	Escherichia coli	[27]
amds	acetamidase	Aspergillus nidulans	[12]
argB	ornithine carbamoyltransferase	Aspergillus nidulans	[12]
nptII	neomycin phosphotransferase II	Escherichia coli	[28]
bar	phosphinothricin acetyltransferase	Streptomyces hygroscopieus	[29]
ptrA	thiamine thiazole synthase	Aspergillus oryzae	[30]
ble	bleomycin binding protein	Streptoalloteichus hindustanus	[31]

ISSN: 1475-2859