Fig. 2From: Current genetic strategies to investigate gene functions in Trichoderma reeseiStrategies for synthesis of siRNA inT. reesei. (a) Identical sequences with target genes are transcribed in both directions under the control of a dual promoter to generate dsRNA. (b) The shRNA is generated by constructing a cassette in which the promoter drives the transcription of two inverted repeat sequences identical to the target gene with a spacer. (c) A complementary sequence to targeting mRNA is transcribed as antisense ssRNA by constructing a cassette in which the promoter drives a transcription of sequence from the antisense strand. The antisense ssRNA will pair with target mRNA to generate the dsRNA. The dsRNA as well as shRNA will be processed by ribonuclease Dicer to generate siRNA for RNAi inductionBack to article page