Fig. 1
From: Current genetic strategies to investigate gene functions in Trichoderma reesei
NHEJ pathway suppresses HR pathway inT. reesei. NHEJ starts with recognizing and binding the DSBs by the Ku70/80 complex, which serves as a scaffold to recruit repair enzymes Mus53(Lig4) to finish the DNA ends ligation. Because NHEJ pathway is dominant during DSB repair in eukaryotic organisms including T. reesei, the NHEJ defective strains, such as Δku70 and Δmus53, have been developed to increase the rate of HR in T. reesei