Table 1 Bacterial strains and plasmids
Strains or plasmids | Characteristics | Source or reference |
|---|---|---|
Strains | ||
L. plantarum GK502 | Source of cryptic plasmid (NCBI accession number CP102362-CP102363) | This study |
L. plantarum SK156 | Recipient in transformation, erythromycin resistance negative | Chae et al. [16] |
L. johnsonii PF01 | Source of erythromycin resistance gene | Chae et al. [16] |
Escherichia coli DH5α | Recipient in transformation for cloning | TaKaRa (Japan) |
Plasmids | ||
pUC19 | pBR322 replication origin, lacZ, Ampr | TaKaRa (Japan) |
pGK02 | Cryptic plasmid from L. plantarum GK502 | This study |
pUGK2 | pUC19 with pGK02 fragment | This study |
pUGK3 | pUGK2 with Emr | This study |
pUGK4 | pUGK3 with surface display system | This study |
pCB4270B-sfGFP | Source of reporter gene, super folder green fluorescent protein (sfGFP) | Jang et al. [20] |
pUGK4-sfGFP | pULP4 with sfGFP gene from pCB4270B-sfGFP in surface display system | This study |
pUGK4-Epitope | pULP4 with SARS-CoV-2 antigen in surface display system | This study |