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Table 1 Production of Trx-KGF-1135 using fed-batch fermentation

From: Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli

KGF

Endpoint OD600

Dry cell weight (g/L)

(wet cell) (g)

Expression level (%)a

Fusion protein (tag-free protein) (g/L)

Solubility (%)

KGF-1135

63.0 ± 0.0

22.1

(185.6)

5.7 ± 0.2

0.6

(0.4)

100

  1. Data are shown as mean ± standard deviation from duplicate experiments
  2. aExpression level and solubility were analyzed by densitometry using ImageJ with duplicate experiments
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Microbial Cell Factories

ISSN: 1475-2859

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