Fig. 4From: Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coliPurification and analysis of KGF-1 135. (a) Overall process of KGF-1135 purification. (b) SDS-PAGE of each purification step. (c) C18 RP-HPLC and (d) LC-MS (Q-TOF) analyses of purified KGF-1135. M, marker; Lys, supernatant after sonication; 1, HisTrap purification; 2, after EK treatment; 3, HiTrap SP purification (final product)Back to article page