Fig. 3
From: Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli
Construction of fusion proteins and expression tests in shake flask culture and fed-batch fermentation. (a) KGF-1135 was fused with TRX via the D4K. (b) The KGF-1 135 fusion protein was expressed in flask culture at 25, 30, and 37 °C. (c) Fed-batch fermentation and expression profiles of TRX-6 H-KGF-1135. T, total fraction; S, soluble fraction; I, insoluble fraction; M, protein marker; Inlet image of SDS-PAGE indicates the expression levels of fusion proteins