Fig. 1
From: Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli
Expression and purification of full-length KGF-1 using E. coli. (a) Construction of KGF-1 fusion protein connected via the EK cleaved site (D4K). (b) Expression of the KGF-1 fusion protein in various temperatures. (c) Purification of KGF-1 with HisTrap and HiTrap SP. (d, e) LC-MS/MS analysis results of purified KGF-1. (f) N-terminal sequence data of purified KGF-1. T, total fraction; S, soluble fraction; I, insoluble fraction; M, protein marker; UB, unbound fraction (flow-through fraction); Black, target sequences; yellow, known as a highly stable sequence; green, the smallest sequence