Fig. 4

(A) The heat map profile of differentially expressed genes, esterase, phthalate metabolic gene (pht) cluster I and II. C protocatechuate metabolic gene (pca) cluster I and II and oxidoreductase. The catabolic gene levels are in the columns and the expression of respective genes is in the rows. Columns are mean-centered, with relative abundance represented by color (green to red from lower to higher abundance), as indicated in the horizontal legend bar. Cross sign indicates absence of gene. (B) RT-qPCR analysis of mRNA transcripts of DEHP-degrading catabolic genes obtained from DEHP-grown cells of strain MBM. Relative changes in gene expression were depicted with succinate-grown cells as control, normalized to 16S rRNA. Mean values were obtained from triplicate measurements