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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.

Fig. 2

Spectral changes during metabolism of protocatechuic acid (PCA) by the cell-free extract of di-(2-ethylhexyl) phthalate (DEHP)-grown cells of strain MBM. The sample and reference cuvettes contained 50 mM potassium phosphate buffer (pH 7.0) in 1-mL volume. The sample cuvette contained 97 nmol of PCA. Spectra were recorded at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 min after the addition 100 µg of crude protein to both cuvettes. Down arrow indicates decreasing absorbance with time

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