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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.

Fig. 1

(A) Growth of Mycolicibacterium sp. strain MBM in mineral salt medium (MSM) upon utilization of di(2-ethylhexyl) phthalate (DEHP) as the sole carbon source under optimal growth conditions in relation to the production of mono(2-ethylhexyl) phthalate (MEHP) and phthalic acid (PA) as transient intermediates. Vertical bars represent mean ± standard deviations from triplicate measurements. (B) HPLC profile of di(2-ethylhexyl) phthalate (DEHP) and its metabolic intermediates from the organic extract of the spent culture of strain MBM incubated with DEHP for 28 h. Inset, UV–visible spectra of peaks obtained with diode array analysis. (C) GC–MS chromatogram and mass fragmentation patterns for the metabolites of DEHP obtained from the organic extract of the resting cell incubation (30 min) of strain MBM. Peak I, 2-ethylhexanol (2-EH); peak II, phthalic acid (PA); peak III, mono(2-ethylhexyl) phthalate (MEHP) and peak IV, di(2-ethylhexyl) phthalate (DEHP)

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