Fig. 5From: Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stressEstablishment of the Type I-F CRISPR-based genome engineering system for Z. mobilis. A A self-targeting plasmid contained an artificial CRISPR locus. B Design of the self-targeting CRISPR and the donor DNA in knockout plasmids; C deletion of mutants by screening of colony PCR. D Confirmation by Sanger sequencingBack to article page