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Table 1 Optimization of CCM in eukaryotic chassis

From: Advances in the optimization of central carbon metabolism in metabolic engineering

Host

Manipulation

CCM involved

Products

References

S. cerevisiae

Introduction of PHK pathway

Glycolysis and PPP

Farnesene

[23]

S. cerevisiae

Introduction of PHK pathway

Glycolysis and PPP

Polyhydroxybutyrate

[25]

S. cerevisiae

Introduction of PHK pathway

Glycolysis and PPP

p-Hydroxycinnamic acid

[30]

S. cerevisiae

Introduction of PHK pathway and A-ALD

Glycolysis and PPP

β-Amyrin

[38]

S. cerevisiae

Introduction of PHK pathway,

overexpression of ADH2, ALD6 and acsSEL641P

Glycolysis and PPP

Fatty acid ethyl esters

[29]

S. cerevisiae

Introduction of PHK pathway, multi-copy integration of Tal1 and Tkl1

Glycolysis and PPP

Protopanaxadiol

[33]

S. cerevisiae

Introduction of PK pathway

Glycolysis and PPP

Tyrosol and salidroside

[31]

S. cerevisiae

Overexpression of a modified PDH pathway

Glycolysis

Acetyl-CoA

[36]

S. cerevisiae

PDC1 deletion

Glycolysis

Pyruvate and lactate

[39]

S. cerevisiae

PDC1 deletion

Glycolysis

2,3-Butanediol

[40]

S. cerevisiae

Deletion of HXK2 and GLK1, tTA-controlled expression of HXK1

Glycolysis

Gluconate

[41]

S. cerevisiae

Introduction of PHK pathway, downregulation of phosphoglucose isomerase, and overexpression Acc1 and Mcr

PPP

3-Hydroxypropionic acid

[34]

S. cerevisiae

Repression of Ric1 and overexpression of Rki1

PPP

Shikimic acid, muconic acid and protocatechuic acid

[46]

S. cerevisiae

Introduction of ACL from A. nidulans

TCA cycle

Mevalonate

[35]

S. cerevisiae

Knockout of 15 genes of CCM

Glycolysis, PPP and TCA cycle

Triacetic acid lactone

[43]

P. pastoris

Overexpression of Zwf, Pgi and Pfk1

Glycolysis

Inositol

[42]

P. pastoris

Control CCM by a synthetic constitutive transcriptional signal amplification device

Glycolysis

Monacolin J

[44]

P. pastoris

Introduction of PHK pathway and the ACL from M. musculus

Glycolysis and TCA cycle

Free fatty acids and fatty alcohol

[28]

Y. lipolytica

Introduction of PHK pathway and PFK deletion

Glycolysis

Lipid

[26]

C. synechocystis

Expression of extra copies of PEPC

TCA cycle

Ethylene

[47]

C. synechocystis

Expression of extra copies of PEPC and elevated culture temperature

Glycolysis and TCA cycle

Succinate

[48]