Table 1 Biosensor design and strain names used in this work. The bidirectional promoters used for expression of heterologous proteins, phytase, laccase, β-casein and β-lactoglobulin fused to GFP11 fragment and the TEV protease fusions to GFP1-10 fragment are indicated.

GFP_AppA

P _CAT1−PGAP

GFP 1–10

+

AppA phytase_GFP11.

GFP_TEV_AppA

P _CAT1−PGAP

GFP 1–10

+

AppA phytase_GFP11.

GFP_PGM1_TEV_AppA

P _CAT1−PGPM1

GFP 1–10

+

AppA phytase_GFP11.

GFP1-11_TEV

P _CAT1−PGAP

GFP 1–11

+

-

GFP1-10_TEV

P _GAP

GFP 1–10

+

-

GFP_TEV_Thi

P _GAP

GFP 1–10

+

Thi laccase_GFP11.

GFP_TEV_ β-cas

P _HpFMD−PGAP

GFP 1–10

+

β-casein_GFP11.

GFP_TEV_ β-lac

P _CAT1−PGAP

GFP 1–10

+

β-lactoglobulin_GFP11.

AppA

P _CAT1

-

-

AppA phytase.

Thi

P _HpFMD

-

-

Thi laccase.