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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris

Fig. 4

Biosensor response during phytase production. a, Biosensor expressed under PGMPI and response during phytase production. b, Biosensor response during phytase production without the TEV protease fusion. Phytase production under methanol induction at 72, 90, 95, 110, 115 and 130 h. Fluorescence is indicated as relative fluorescence units (RFU) at 485–520 nm and phytase activity as absorbance at 405 nm. Correlation between secreted enzyme activity and intracellular GFP fluorescence shown in framed graph. Data are shown as the mean and standard deviation of three biological replicates. The dashed line indicates background fluorescence at 485–520 nm. c, Fluorescence of the biosensor controls. Complete biosensor GFP1-11_TEV protease (GFP1-11_TEV), biosensor fragment GFP1-10_TEV protease (GFP1-10_TEV) and wild type MutS strain (WT).

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