Fig. 2

Biosensor response during phytase and laccase production. a, Phytase and b, laccase production under methanol induction at 72, 90, 95, 110, 115 and 130 h. Fluorescence is indicated as relative fluorescence units (RFU) and phytase and laccase activity as absorbance at 405 and 410 nm, respectively. Correlation between secreted enzyme activity and intracellular GFP fluorescence shown in framed graphs. Data are shown as the mean and standard deviation of three biological replicates and analysed using one-way ANOVA with multiple comparisons. The dashed line indicates background fluorescence at 485–520 nm in wild-type fermentations. *Significantly different to all colonies (p < 0.01). **Significantly different to all colonies except colony 13 at 130 h (p < 0.01). ^# Significantly different all colonies except colonies 2, 3, 4, 5 and 7 (p < 0.01). ^Δ Significantly different all colonies except colonies 2, 3, 4, 5,10 14, 18, 23, 25 and 28 (p < 0.01)