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Fig. 1 | Microbial Cell Factories

Fig. 1

From: High-level production of Aspergillus niger prolyl endopeptidase from agricultural residue and its application in beer brewing

Fig. 1

Plasmid profiles, and heterologous expression of prolyl endopeptidase in T. reesei. A, B Schematic drawing of plasmids pCBH12-AN-PEP and pCBH12-AN-PEP* bearing cbh1 promoter, cbh1 signal peptide, A. niger PEP gene, and cbh2 terminator. AN-PEP: A. niger PEP gene with intron; AN-PEP*: A. niger PEP cDNA. C Activity analysis of prolyl endopeptidase secreted by transformants bearing pCBH12-AN-PEP and pCBH12-AN-PEP*. The error bar denotes the standard deviations (SD) of two biological replicates (ns: not significant). D Activity analysis of the positive transformants pCBH12-AN-PEP fermentation supernatants. The error bar denotes the standard deviations (SD) of three technical repeats (****p < 0.0001). 1–4: positive transformants 1–4; 5: T. reesei TU6

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Microbial Cell Factories

ISSN: 1475-2859

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