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Fig. 5 | Microbial Cell Factories

Fig. 5

From: A novel thermostable TP-84 capsule depolymerase: a method for rapid polyethyleneimine processing of a bacteriophage-expressed proteins

Fig. 5

Determination of the depolymerase presence in TP-84 virions. Panel a SDS-PAGE of structural TP-84 proteins in 10% gel (concentrated virions purified by CsCl density gradient; after single ultracentrifugation). Lane M, PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa; Lane 1, TP-84 bacteriophage, app. 1011 virions. Panel b SDS-PAGE of structural TP-84 proteins in 8% gel (CsCl density gradient; after double ultracentrifugation). Lane M, PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa; Lane 1, TP-84 bacteriophage, app. 4 × 1010 virions. Panel c As in panel c after Western blotting and immunodetection with anti-TP-84 antibodies

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Microbial Cell Factories

ISSN: 1475-2859

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