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Fig. 10 | Microbial Cell Factories

Fig. 10

From: A novel thermostable TP-84 capsule depolymerase: a method for rapid polyethyleneimine processing of a bacteriophage-expressed proteins

Fig. 10

Analysis of TP84_26 depolymerase quaternary composition. Panel a. Purified depolymerase electrophoresis (6% PAGE) under native buffer conditions (non-denaturing). Lane M1, PageRuler™ Plus Prestained Protein Ladder; lane 1, TP84_26 SDS-denatured, 24 μg; lane 2, 12 μg; lane 3, 6 μg; Lane M2, GE Healthcare LMW SDS Marker Kit (not heated); lane 4, TP84_26 native (not denatured), 24 μg; lane 5, 12 μg; lane 6, 6 μg. Panel b. SDS-PAGE of depolymerase peak fractions from Sephacryl S300 HR column, silver stained. Lane M, PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa;  lanes 62 to 68, consecutive Sephacryl S300 HR fractions. Panel c. Spontaneous degradation of depolymerase upon changing pH from 6.0 to 8.0. Panel d. Molecular sieving of depolymerase on Sephacryl S300, calibrated with 5 mg each of the native markers: ferritin, aldolase, conalbumin, ovalbumin, cytochrome C

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