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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity

Fig. 1

Luciferase assay of constitutive promoters in P. stutzeri DSM4166 in the LB medium under the aerobic condition. (A) Schematic of the plasmid containing different promoters. pBBR1: replicon; kan: kanamycin resistance gene; firefly: firefly luciferase reporter gene. (B) Luciferase activity of plasmids containing different promoters in DSM4166. Vector: the promoterless plasmid. Pgenta: the promoter of the gentamicin resistance gene. PnifA: the promoter of the nitrogen fixation pathway specific positive regulator gene nifA. Error bars indicate the standard deviations of three replicates (n = 3). The P-value cutoff for all the plots is 0.05. * P < 0.05, ** P < 0.01, and *** P < 0.001. One-way ANOVA test with Tukey Pairwise comparisons was used to compute statistical significance

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Microbial Cell Factories

ISSN: 1475-2859

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