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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Optimizing yeast for high-level production of kaempferol and quercetin

Fig. 2

Production of kaempferol from different naringenin platform strains. (a) Screening a variety of F3H and FLS from different plant sources to determine the best combination by testing in NAG10 strain, being capable to produce 80 mg L− 1 of naringenin. (b) Investigating the same enzyme combinations in the background of NAG3-4 strain, which has a higher naringenin production (180 mg L−1). AtF3H from Arabidopsis thaliana, OsF3H from Oryza sativa, AtFLS from A. thaliana, PdFLS from Populus deltoides, and VvFLS from Vitis vinifera. The black circle shows the existence of the gene in the strain, instead of the white circle representing its absence. Cells were cultured in a defined minimal medium in shake flask conditions, and samples were taken after 72 h of growth for naringenin, dihydrokaempferol, and kaempferol detection. Statistical analysis was performed by using one-way ANOVA, Tukey’s multiple comparison test * < 0.05, ** < 0.01, *** < 0.001). All data represent the mean ± standard deviation of 3 biologically independent samples

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