Enhanced supply of acetyl-CoA by exogenous pantothenate kinase promotes synthesis of poly(3-hydroxybutyrate)

Table 1 Strains and plasmids used in this work

Strain or plasmid	Relevant genotype or characteristic	Source or reference
Strain
Pseudomonas putida JCM 20,089	Template DNA for PCR	JCM^a
Microcystis aeruginosa NIES-843	Template DNA for PCR	NIES^b
Escherichia coli JM109	recA1, endA1, gyrA96, thi-1, hsdR17(r_k⁻m_k⁺), e14⁻(mcrA⁻), supE44, relA1, Δ(lac-proAB)/F'[traD36, proAB⁺, lacI^q, lacZΔM15]	Takara
Plasmid
pQE-60	Ap^r, ColE1, expression vector, T5 promoter	Qiagen
pQE-Ma-phaABEC	Ap^r, pQE-60 carrying pha gene cluster of phaA, phaB, phaE and phaC from M. aeruginosa under T5 promoter	This work
pSTV28	Cm^r, p15A, cloning vector, lac promoter	Takara
pSTV-Pp-coaA	Cm^r, pSTV28 carrying coaA from P. putida under lac promoter	12

ISSN: 1475-2859