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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Enhanced supply of acetyl-CoA by exogenous pantothenate kinase promotes synthesis of poly(3-hydroxybutyrate)

Fig. 5

Accumulation of P(3HB) by enhanced acetyl-CoA supply in E. coli. The transformants of E. coli JM109/pSTV28 + pQE-Ma-phaABEC (closed symbols) and JM109/pSTV-Pp-coaA + pQE-Ma-phaABEC (open symbols) were aerobically cultivated in 150 mL of M9 minimal medium in the absence (circles) or presence of 2 mM pantothenate (triangles) or 2 mM β-alanine (squares) for 120 h at 37 °C. Panels A–D show P(3HB) content and cell mass as described in Fig. 3. Panels E, F, and G indicate residual glucose, pH, and acetate in the culture media, respectively. The significant differences among the six experimental groups at 120 h of cultivation were analyzed by Turkey’s HSD test (p < 0.05). All data are shown as the mean ± SD (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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