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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Enhanced supply of acetyl-CoA by exogenous pantothenate kinase promotes synthesis of poly(3-hydroxybutyrate)

Fig. 2

P(3HB) contents in E. coli cells carrying the pha genes from M. aeruginosa. The transformants of E. coli JM109/pSTV28 + pQE-Ma-phaABEC and JM109/pSTV-Pp-coaA + pQE-Ma-phaABEC were cultivated aerobically in 30 mL of M9 minimal medium supplemented with 5 mM pantothenate and 2% (w/v) glucose in the absence (solid bar) or presence (open bar) of 0.1 mM IPTG for 48 h at 30 °C (A) and 37 °C (B). P(3HB) content was determined as 3HB methyl esters using GC-FID. The significance of the differences was analyzed by Tukey's HSD test (p < 0.01). The data are shown as the mean ± SD (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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