Fig. 5
scFvCD16A-sc4-1BBL stimulated the proliferation of PBMC-derived NK cells, and the expanded NK cells were cytotoxic to K562 cells. 1.5 X 106 PBMCs from healthy donors (n = 6) were cultured in KBM581 medium with 2 μg/ml scFvCD16A-sc4-1BBL precoated on the flask for 13 days and supplemented with rhIL-2 ( 1000 IU/ml). a The curves show the counts of NK cells and total cells. b The increased folds of NK cell number and total cell number. c The proportion of NK cells. d The cytotoxicity of expanded NK cells was analysed by a killing assay, and the curve shows the specific killing percentage of expanded NK cells targeting K562 cells at different E:T ratios. The data are shown as the mean ± SD from 6 independent experiments