Fig. 4

scFvCD16A-sc4-1BBL activated PBMC-derived NK cells. Human PBMCs from healthy donors (n = 5) were cultured in 1640 media for 24 h in the presence of 50 IU/mL rhIL-2, 20 nM scFvCD16A-sc4-1BBL, 20 nM scFvCD16A, 60 nM mn4-1BBL or 20 nM scFvCD16A + 60 nM mn4-1BBL, respectively. The expression of the indicated protein markers on NK cells (CD3⁻CD56⁺) and CD8 + T cells was analysed by flow cytometry. a Cell proportions of different immune cells under the indicated treatments. b The expression levels of IFN-γ, Granzyme, Perforin, Ki67 in CD8⁺ T cells. c Heatmap showing the expression levels of surface receptors and intracellular granule components in NK cells under the indicated treatments. Datas are represented as the mean ± SD from 5 independent experiments. ns: not significant; * p < 0.05; ** p < 0.01