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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Development of an antibody-ligand fusion protein scFvCD16A-sc4-1BBL in Komagataella phaffii with stimulatory activity for Natural Killer cells

Fig. 3

Binding properties of scFvCD16A-sc4-1BBL to its receptors. The affinity of scFvCD16A-sc4-1BBL to human CD16A and 4-1BB was analyzed by ELISA (a) and Surface Plasmon Resonance (SPR) (b and c). The concentrations of the tested proteins are indicated. a. The curves are ELISA results showing the binding of scFvCD16A-sc4-1BBL to human CD16A receptor (top panel) or human 4-1BB receptor (bottom panel), all the points are shown as the mean ± SD from three independent assays, n = 3. b The scFvCD16A-sc4-1BBL (top panel) or the reference protein scFvCD16A (bottom panel) binds to human CD16A, and the table shows the analysis results from the figure. c. The scFvCD16A-sc4-1BBL (top panel) or the control protein mn4-1BBL (bottom panel) binds to the human 4-1BB receptor, and the table shows the analysis results from the figure. Ka: association rate constant; Kd: dissociation rate constant; KD (= Kd/Ka): equilibrium dissociation (binding) rate constant; EC50: concentration to reach half of the maximal binding

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Microbial Cell Factories

ISSN: 1475-2859

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