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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography

Fig. 5

Binding of H6-tagged recombinant proteins to Ni2+ columns. A Western Blot (WB) of protein samples purified by the traditional protocol or assisted by N-L. For each protein, the binding efficiency of the proteins to the Ni-column is shown through the flow through (FT) and the wash (W) sample analyses. B General architecture of the fusion proteins used here to determine final yield. Box sizes were only indicative. C Summary of yield and purity data

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Microbial Cell Factories

ISSN: 1475-2859

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