Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography

Fig. 3

Comparative purification of cationic modular proteins based on INFγ. A General architecture of the fusion proteins used here. Box sizes were only indicative. B Purity data summary. C–E Chromatographic profiles for affinity chromatography of GWH1-INFγ-H6 (C), PAD-INFγ-H6 (D) and SPR2-INFγ-H6 from the E. coli soluble cell fraction in absence or presence of N-L in the cell extracts. Vertical dashed lines and numbers indicate the particular protein fraction selected for further analysis. The plots are sided by SDS-PAGE coupled to TGX stain free gel Technology (Bio-Rad) of protein fractions indicated by vertical lines

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us