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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography

Fig. 2

Comparative purification of cationic modular proteins based on GFP. A General architecture of the fusion proteins used here. Box sizes were only indicative. B Purity data summary. C and D Chromatographic profiles for affinity chromatography of PAD-GFP-H6 (C) and SPR2-GFP-H6 (D) from the E. coli soluble cell fraction in absence or presence of N-L in the cell extracts. Vertical dashed lines and numbers indicate the fraction selected for further analysis. The plots are sided by SDS-PAGE coupled to TGX stain free gel Technology (Bio-Rad) of protein fractions indicated in vertical lines

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Microbial Cell Factories

ISSN: 1475-2859

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