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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography

Fig. 1

Purification of GFP-H6 and GWH1-GFP-H6. A Modular architecture of GFP-H6 and GWH1-GFP-H6. Box sizes are only approximate. B Chromatographic profile for affinity chromatography of GFP-H6 and GWH1-GFP-H6 constructs present in the E. coli soluble cell fraction in the absence or presence of different detergents. Vertical dashed lines and numbers indicate the fraction selected for further analysis. C SDS-PAGE coupled to TGX stain-free gel Technology (Bio-Rad) of the protein fractions indicated through vertical lines in the panel B. D DLS size determination of GWH1-GFP-H6 purified in the presence of alternative detergents. Numbers indicate peak sizes. E Temperature-dependent DLS size data of GWH1-GFP-H6 purified in the presence of alternative detergents. F Circular Dichroism (CD) spectra of GWH1-GFP-H6 protein samples purified in the presence of alternative detergents. G GFP fluorescence spectrum of GWH1-GFP-H6 purified in the presence of alternative detergents

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