Table 2 Hydrolytic substrate specificity of MsAA. Substrates were used at 15 mM concentration in 100 mM Tris-HCl pH 7.0. Reaction temperature was elevated to 50 °C to ensure solubilization of all substrates
Substrate | Activity [U/mg] |
|---|---|
Acetyl-alanine | 265.9 (± 7.8) |
Benzoyl-alanine | 2.7 ± (0.1) |
Lauroyl-alanine | 20.6 (± 1.4) |
Palmitoyl-alanine | 20.7 (± 0.8) |
Acetyl-methionine | 140.9 (± 6.6) |
Lauroyl-methionine | 54.9 (± 0.7) |
Acetyl-valine | 326.1 (± 5,7) |
Lauroyl-valine | 7.1 (± 0.8) |
Acetyl-isoleucine | 284.2 (± 13.4) |
Lauroyl-isoleucine | 2.2 (± 0.3) |
Acetyl-glutamine | 44.6 (± 1.7) |
Octanoyl-glutamine | 0.8 (± 0.1) |
Lauroyl-glutamine | 0 |
Palmitoyl-glutamine | 0 |
Acetyl-leucine | 147.1 (± 6.8) |
Lauroyl-leucine | 0.5 (± 0.1) |
Acetyl-threonine | 63.5 (± 2.1) |
Acetyl-aspartic acid | 41.0 (± 0.6) |
Lauroyl-aspartic acid | 0 |
Acetyl-glycine | 89.6 (± 1.8) |
Lauroyl-glycine | 2.7 (± 0.1) |
Acetyl-glutamic acid | 27.5 (± 1.4) |
Acetyl-arginine | 17.5 (± 0.6) |
Acetyl-asparagine | 20.3 (± 0.7) |
Acetyl-cysteine | 39.4 (± 2.5) |
Lauroyl-cysteine | 0 |
Acetyl-phenylalanine | 3.4 (± 0.2) |
Lauroyl-phenylalanine | 0 |
Acetyl-tyrosine | 1.2 (± 0.1) |
Lauroyl-tyrosine | 0 |
Nα-acetyl-lysine | 18.1 (± 0.6) |
Nε-acetyl-lysine | 0 |
Acetyl-tryptophane | 0 |
Lauroyl-tryptophane | 0 |
Acetyl-proline | 0 |