Fig. 5From: Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesisSDS-PAGE of MsAA NTag overexpression and Strep-tag purification from E. coli ArcticExpress (DE3). Lane 1: Protein marker (BlueEasy Prestained Protein Marker, Nippon Genetics); lane 2: cell-free extract; lane 3: insoluble fraction; lane 4: Flow-through; lane 5/6: wash fractions; lane 7: Elution of MsAA NTag.Back to article page