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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis

Fig. 1

Multiple sequence alignment of MsAA and homologous proteins

MsAA from M. smegmatis (AWT55079.1), the aminoacylases SamAA from S. ambofaciens (AKZ54783.1), SmAA from S. mobaraensis (BAI44523.1), and DapE from Haemophilus influenzae (WP_005693818.1) are shown. The alignment generated using T-Coffee and displayed with ESPript 3.0. The conserved metal-binding residues (H91, D123, E158, E185, H425) and catalytic residues (D93, E157, H226) are highlighted by green and blue boxes with asterisks. The annotation of the secondary structural elements shown above is based on the ColabFold-generated structure of MsAA; the secondary structural elements shown below belong to DapE (imported from PDB-entry 5vo3). The secondary structure is displayed with arrows for β-strands, squiggles for α-helices or 310(η)-helices and the letters TT for turns

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Microbial Cell Factories

ISSN: 1475-2859

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